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6lpc

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==Crystal Structure of rat Munc18-1 with K332E/K333E mutation==
==Crystal Structure of rat Munc18-1 with K332E/K333E mutation==
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<StructureSection load='6lpc' size='340' side='right'caption='[[6lpc]]' scene=''>
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<StructureSection load='6lpc' size='340' side='right'caption='[[6lpc]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LPC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LPC FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6lpc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LPC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LPC FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lpc OCA], [http://pdbe.org/6lpc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lpc RCSB], [http://www.ebi.ac.uk/pdbsum/6lpc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lpc ProSAT]</span></td></tr>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Stxbp1, Unc18a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lpc OCA], [http://pdbe.org/6lpc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lpc RCSB], [http://www.ebi.ac.uk/pdbsum/6lpc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lpc ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/STXB1_RAT STXB1_RAT]] May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Priming of synaptic vesicles involves Munc13-catalyzed transition of the Munc18-1/syntaxin-1 complex to the SNARE complex in the presence of SNAP-25 and synaptobrevin-2; Munc13 drives opening of syntaxin-1 via the MUN domain while Munc18-1 primes SNARE assembly via domain 3a. However, the underlying mechanism remains unclear. In this study, we have identified a number of residues in domain 3a of Munc18-1 that are crucial for Munc13 and Munc18-1 actions in SNARE complex assembly and synaptic vesicle priming. Our results showed that two residues (Q301/K308) at the side of domain 3a mediate the interaction between the Munc18-1/syntaxin-1 complex and the MUN domain. This interaction enables the MUN domain to drive the opening of syntaxin-1 linker region, thereby leading to the extension of domain 3a and promoting synaptobrevin-2 binding. In addition, we identified two residues (K332/K333) at the bottom of domain 3a that mediate the interaction between Munc18-1 and the SNARE motif of syntaxin-1. This interaction ensures Munc18-1 to persistently associate with syntaxin-1 during the conformational change of syntaxin-1 from closed to open, which reinforces the role of Munc18-1 in templating SNARE assembly. Taken together, our data suggest a mechanism by which Munc13 activates the Munc18-1/syntaxin-1 complex and enables Munc18-1 to prime SNARE assembly.
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Munc13 activates the Munc18-1/syntaxin-1 complex and enables Munc18-1 to prime SNARE assembly.,Wang X, Gong J, Zhu L, Wang S, Yang X, Xu Y, Yang X, Ma C EMBO J. 2020 Jul 9:e103631. doi: 10.15252/embj.2019103631. PMID:32643828<ref>PMID:32643828</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6lpc" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Buffalo rat]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Gong JH]]
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[[Category: Gong, J H]]
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[[Category: Ma C]]
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[[Category: Ma, C]]
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[[Category: Wang S]]
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[[Category: Wang, S]]
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[[Category: Wang XP]]
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[[Category: Wang, X P]]
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[[Category: Xu YY]]
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[[Category: Xu, Y Y]]
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[[Category: Yang XF]]
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[[Category: Yang, X F]]
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[[Category: Yang XY]]
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[[Category: Yang, X Y]]
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[[Category: Zhu L]]
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[[Category: Zhu, L]]
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[[Category: Exocytosis]]
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[[Category: Membrane fusion]]
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[[Category: Snares-binding]]
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[[Category: Synaptic exocytosis]]

Revision as of 07:01, 2 September 2020

Crystal Structure of rat Munc18-1 with K332E/K333E mutation

PDB ID 6lpc

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