6lpc
From Proteopedia
(Difference between revisions)
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==Crystal Structure of rat Munc18-1 with K332E/K333E mutation== | ==Crystal Structure of rat Munc18-1 with K332E/K333E mutation== | ||
| - | <StructureSection load='6lpc' size='340' side='right'caption='[[6lpc]]' scene=''> | + | <StructureSection load='6lpc' size='340' side='right'caption='[[6lpc]], [[Resolution|resolution]] 3.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LPC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LPC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6lpc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LPC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LPC FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lpc OCA], [http://pdbe.org/6lpc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lpc RCSB], [http://www.ebi.ac.uk/pdbsum/6lpc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lpc ProSAT]</span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Stxbp1, Unc18a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lpc OCA], [http://pdbe.org/6lpc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lpc RCSB], [http://www.ebi.ac.uk/pdbsum/6lpc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lpc ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/STXB1_RAT STXB1_RAT]] May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Priming of synaptic vesicles involves Munc13-catalyzed transition of the Munc18-1/syntaxin-1 complex to the SNARE complex in the presence of SNAP-25 and synaptobrevin-2; Munc13 drives opening of syntaxin-1 via the MUN domain while Munc18-1 primes SNARE assembly via domain 3a. However, the underlying mechanism remains unclear. In this study, we have identified a number of residues in domain 3a of Munc18-1 that are crucial for Munc13 and Munc18-1 actions in SNARE complex assembly and synaptic vesicle priming. Our results showed that two residues (Q301/K308) at the side of domain 3a mediate the interaction between the Munc18-1/syntaxin-1 complex and the MUN domain. This interaction enables the MUN domain to drive the opening of syntaxin-1 linker region, thereby leading to the extension of domain 3a and promoting synaptobrevin-2 binding. In addition, we identified two residues (K332/K333) at the bottom of domain 3a that mediate the interaction between Munc18-1 and the SNARE motif of syntaxin-1. This interaction ensures Munc18-1 to persistently associate with syntaxin-1 during the conformational change of syntaxin-1 from closed to open, which reinforces the role of Munc18-1 in templating SNARE assembly. Taken together, our data suggest a mechanism by which Munc13 activates the Munc18-1/syntaxin-1 complex and enables Munc18-1 to prime SNARE assembly. | ||
| + | |||
| + | Munc13 activates the Munc18-1/syntaxin-1 complex and enables Munc18-1 to prime SNARE assembly.,Wang X, Gong J, Zhu L, Wang S, Yang X, Xu Y, Yang X, Ma C EMBO J. 2020 Jul 9:e103631. doi: 10.15252/embj.2019103631. PMID:32643828<ref>PMID:32643828</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6lpc" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Buffalo rat]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Gong | + | [[Category: Gong, J H]] |
| - | [[Category: Ma C]] | + | [[Category: Ma, C]] |
| - | [[Category: Wang S]] | + | [[Category: Wang, S]] |
| - | [[Category: Wang | + | [[Category: Wang, X P]] |
| - | [[Category: Xu | + | [[Category: Xu, Y Y]] |
| - | [[Category: Yang | + | [[Category: Yang, X F]] |
| - | [[Category: Yang | + | [[Category: Yang, X Y]] |
| - | [[Category: Zhu L]] | + | [[Category: Zhu, L]] |
| + | [[Category: Exocytosis]] | ||
| + | [[Category: Membrane fusion]] | ||
| + | [[Category: Snares-binding]] | ||
| + | [[Category: Synaptic exocytosis]] | ||
Revision as of 07:01, 2 September 2020
Crystal Structure of rat Munc18-1 with K332E/K333E mutation
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Categories: Buffalo rat | Large Structures | Gong, J H | Ma, C | Wang, S | Wang, X P | Xu, Y Y | Yang, X F | Yang, X Y | Zhu, L | Exocytosis | Membrane fusion | Snares-binding | Synaptic exocytosis
