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| | <StructureSection load='6t1p' size='340' side='right'caption='[[6t1p]], [[Resolution|resolution]] 3.50Å' scene=''> | | <StructureSection load='6t1p' size='340' side='right'caption='[[6t1p]], [[Resolution|resolution]] 3.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6t1p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"ascococcus_mesenteroides"_tsenkovskii_1878 "ascococcus mesenteroides" tsenkovskii 1878]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T1P OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6T1P FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6t1p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leuconostoc_mesenteroides Leuconostoc mesenteroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T1P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6T1P FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">asr ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1245 "Ascococcus mesenteroides" Tsenkovskii 1878])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alternansucrase Alternansucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.140 2.4.1.140] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6t1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t1p OCA], [https://pdbe.org/6t1p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6t1p RCSB], [https://www.ebi.ac.uk/pdbsum/6t1p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6t1p ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6t1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t1p OCA], [http://pdbe.org/6t1p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6t1p RCSB], [http://www.ebi.ac.uk/pdbsum/6t1p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6t1p ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9RE05_LEUME Q9RE05_LEUME] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ascococcus mesenteroides tsenkovskii 1878]] | |
| - | [[Category: Alternansucrase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Cioci, G]] | + | [[Category: Leuconostoc mesenteroides]] |
| - | [[Category: Molina, M]] | + | [[Category: Cioci G]] |
| - | [[Category: Moulis, C]] | + | [[Category: Molina M]] |
| - | [[Category: Remaud-Simeon, M]] | + | [[Category: Moulis C]] |
| - | [[Category: Alternan]]
| + | [[Category: Remaud-Simeon M]] |
| - | [[Category: Dextran]]
| + | |
| - | [[Category: Gh70]]
| + | |
| - | [[Category: Glucansucrase]]
| + | |
| - | [[Category: Polysaccharide]]
| + | |
| - | [[Category: Sucrose]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
Q9RE05_LEUME
Publication Abstract from PubMed
Microbial alpha-glucans produced by glycoside hydrolase family 70 (GH70) glucansucrases are gaining importance because of the mild conditions for their synthesis from sucrose, their biodegradability, and their current and anticipated applications that largely depend on their molar mass. Focusing on the alternansucrase (ASR) from Leuconostoc citreum NRRL B-1355, a well-known glucansucrase catalyzing the synthesis of both high-molar mass (HMM) and low-molar mass alternans, we searched for structural traits in ASR that could be involved in the control of alternan elongation. The resolution of five crystal structures of a truncated ASR version (ASRDelta2) in complex with different gluco-oligosaccharides pinpointed key residues in binding sites located in the A and V domains of ASR. Biochemical characterization of three single mutants and three double mutants, targeting the sugar-binding pockets identified in domain V, revealed an involvement of this domain in alternan binding and elongation. More strikingly, we found an oligosaccharide-binding site at the surface of domain A, distant from the catalytic site and not previously identified in other glucansucrases. We named this site surface binding site A1 (SBS-A1). Among the residues lining the SBS-A1 site, two (Gln-700 and Tyr-717) promoted alternan elongation. Their substitution to alanine decreased HMM alternan yield by a third, without significantly impacting enzyme stability or specificity. We propose that the SBS-A1 site is unique to alternansucrase and appears to be designed to bind alternating structures, acting as a mediator between the catalytic site and the sugar-binding pockets of domain V and contributing to a processive elongation of alternan chains.
A specific oligosaccharide-binding site in alternansucrase catalytic domain mediates alternan elongation.,Molina M, Moulis C, Monties N, Guieysse D, Morel S, Cioci G, Remaud-Simeon M J Biol Chem. 2020 May 14. pii: RA120.013028. doi: 10.1074/jbc.RA120.013028. PMID:32409580[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Molina M, Moulis C, Monties N, Guieysse D, Morel S, Cioci G, Remaud-Simeon M. A specific oligosaccharide-binding site in alternansucrase catalytic domain mediates alternan elongation. J Biol Chem. 2020 May 14. pii: RA120.013028. doi: 10.1074/jbc.RA120.013028. PMID:32409580 doi:http://dx.doi.org/10.1074/jbc.RA120.013028
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