6m48
From Proteopedia
(Difference between revisions)
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==Crystal structure of pilus adhesin, SpaC from Lactobacillus rhamnosus GG - P21212 form== | ==Crystal structure of pilus adhesin, SpaC from Lactobacillus rhamnosus GG - P21212 form== | ||
- | <StructureSection load='6m48' size='340' side='right'caption='[[6m48]]' scene=''> | + | <StructureSection load='6m48' size='340' side='right'caption='[[6m48]], [[Resolution|resolution]] 2.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
- | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M48 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6M48 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6m48]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lacrg Lacrg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M48 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6M48 FirstGlance]. <br> |
- | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6m48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m48 OCA], [http://pdbe.org/6m48 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m48 RCSB], [http://www.ebi.ac.uk/pdbsum/6m48 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m48 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
+ | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6m3y|6m3y]]</td></tr> | ||
+ | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LGG_00444 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=568703 LACRG])</td></tr> | ||
+ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6m48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m48 OCA], [http://pdbe.org/6m48 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m48 RCSB], [http://www.ebi.ac.uk/pdbsum/6m48 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m48 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | Adhesion to cell surfaces is an essential and early prerequisite for successful host colonization by bacteria, and in most instances involves the specificities of various adhesins. Among bacterial Gram-positives, some genera and species mediate attachment to host cells by using long non-flagellar appendages called sortase-dependent pili. A case in point is the beneficial Lactobacillus rhamnosus GG gut-adapted strain that produces the so-called SpaCBA pilus, a structure noted for its promiscuous binding to intestinal mucus and collagen. Structurally, SpaCBA pili are heteropolymers of three different pilin-protein subunits, each with its own location and function in the pilus: backbone SpaA for length, basal SpaB for anchoring, and tip SpaC for adhesion. Previously, we solved the SpaA tertiary structure by X-ray crystallography and also reported on the crystallization of SpaB and SpaC. Here, we reveal the full-length high-resolution (1.9 A) crystal structure of SpaC, a first for a sortase-dependent pilus-bearing commensal. The SpaC structure, unlike the representative four-domain architecture of other Gram-positive tip pilins, espouses an atypically longer five-domain arrangement that includes N-terminal 'binding' and C-terminal 'stalk' regions of two and three domains, respectively. With the prospect of establishing new mechanistic insights, we provide a structural basis for the multi-substrate binding nature of SpaC, as well as a structural model that reconciles its exclusive localization at the SpaCBA pilus tip. | ||
+ | |||
+ | Crystal structure of lactobacillar SpaC reveals an atypical five-domain pilus tip adhesin: Exposing its substrate-binding and assembly in SpaCBA pili.,Kant A, Palva A, von Ossowski I, Krishnan V J Struct Biol. 2020 Jul 9:107571. doi: 10.1016/j.jsb.2020.107571. PMID:32653644<ref>PMID:32653644</ref> | ||
+ | |||
+ | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
+ | </div> | ||
+ | <div class="pdbe-citations 6m48" style="background-color:#fffaf0;"></div> | ||
+ | == References == | ||
+ | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
+ | [[Category: Lacrg]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
- | [[Category: Kant A]] | + | [[Category: Kant, A]] |
- | [[Category: Krishnan V]] | + | [[Category: Krishnan, V]] |
- | [[Category: Palva A]] | + | [[Category: Ossowaski, I Von]] |
- | [[Category: | + | [[Category: Palva, A]] |
+ | [[Category: Cell adhesion]] | ||
+ | [[Category: Fimbria]] | ||
+ | [[Category: Lactobacillus rhamnosus gg]] | ||
+ | [[Category: Lectin]] | ||
+ | [[Category: Mida]] | ||
+ | [[Category: Pili]] | ||
+ | [[Category: Pilus adhesin]] | ||
+ | [[Category: Probiotic]] | ||
+ | [[Category: Sortase]] | ||
+ | [[Category: Spac]] | ||
+ | [[Category: Spacba pilus]] | ||
+ | [[Category: Surface protein]] | ||
+ | [[Category: Tip pilin]] | ||
+ | [[Category: Vwfa domain]] |
Revision as of 06:31, 19 August 2020
Crystal structure of pilus adhesin, SpaC from Lactobacillus rhamnosus GG - P21212 form
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Categories: Lacrg | Large Structures | Kant, A | Krishnan, V | Ossowaski, I Von | Palva, A | Cell adhesion | Fimbria | Lactobacillus rhamnosus gg | Lectin | Mida | Pili | Pilus adhesin | Probiotic | Sortase | Spac | Spacba pilus | Surface protein | Tip pilin | Vwfa domain