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| | <StructureSection load='6tl8' size='340' side='right'caption='[[6tl8]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='6tl8' size='340' side='right'caption='[[6tl8]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6tl8]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TL8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TL8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6tl8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TL8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TL8 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Lrfn4, Salm3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tl8 OCA], [http://pdbe.org/6tl8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tl8 RCSB], [http://www.ebi.ac.uk/pdbsum/6tl8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tl8 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tl8 OCA], [https://pdbe.org/6tl8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tl8 RCSB], [https://www.ebi.ac.uk/pdbsum/6tl8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tl8 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CD33_HUMAN CD33_HUMAN]] Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).<ref>PMID:10556798</ref> <ref>PMID:11320212</ref> | + | [https://www.uniprot.org/uniprot/LRFN4_MOUSE LRFN4_MOUSE] Promotes neurite outgrowth in hippocampal neurons. May play a role in redistributing DLG4 to the cell periphery.<ref>PMID:16828986</ref> <ref>PMID:18585462</ref> [https://www.uniprot.org/uniprot/CD33_HUMAN CD33_HUMAN] Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).<ref>PMID:10556798</ref> <ref>PMID:11320212</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bae, S]] | + | [[Category: Mus musculus]] |
| - | [[Category: Kajander, T]] | + | [[Category: Bae S]] |
| - | [[Category: Karki, S]] | + | [[Category: Kajander T]] |
| - | [[Category: Ko, J]] | + | [[Category: Karki S]] |
| - | [[Category: Shkumatov, A V]] | + | [[Category: Ko J]] |
| - | [[Category: Cell adhesion]]
| + | [[Category: Shkumatov AV]] |
| - | [[Category: Leucine rich repeat]]
| + | |
| - | [[Category: Salm3]]
| + | |
| - | [[Category: Synapse]]
| + | |
| Structural highlights
Function
LRFN4_MOUSE Promotes neurite outgrowth in hippocampal neurons. May play a role in redistributing DLG4 to the cell periphery.[1] [2] CD33_HUMAN Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).[3] [4]
Publication Abstract from PubMed
Synaptic adhesion molecules play an important role in the formation, maintenance and refinement of neuronal connectivity. Recently, several leucine rich repeat (LRR) domain containing neuronal adhesion molecules have been characterized including netrin G-ligands, SLITRKs and the synaptic adhesion-like molecules (SALMs). Dysregulation of these adhesion molecules have been genetically and functionally linked to various neurological disorders. Here we investigated the molecular structure and mechanism of ligand interactions for the postsynaptic SALM3 adhesion protein with its presynaptic ligand, receptor protein tyrosine phosphatase sigma (PTPsigma). We solved the crystal structure of the dimerized LRR domain of SALM3, revealing the conserved structural features and mechanism of dimerization. Furthermore, we determined the complex structure of SALM3 with PTPsigma using small angle X-ray scattering, revealing a 2:2 complex similar to that observed for SALM5. Solution studies unraveled additional flexibility for the complex structure, but validated the uniform mode of action for SALM3 and SALM5 to promote synapse formation. The relevance of the key interface residues was further confirmed by mutational analysis with cellular binding assays and artificial synapse formation assays. Collectively, our results suggest that SALM3 dimerization is a pre-requisite for the SALM3-PTPsigma complex to exert synaptogenic activity.
Structural basis of SALM3 dimerization and synaptic adhesion complex formation with PTPsigma.,Karki S, Shkumatov AV, Bae S, Kim H, Ko J, Kajander T Sci Rep. 2020 Jul 14;10(1):11557. doi: 10.1038/s41598-020-68502-4. PMID:32665594[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Morimura N, Inoue T, Katayama K, Aruga J. Comparative analysis of structure, expression and PSD95-binding capacity of Lrfn, a novel family of neuronal transmembrane proteins. Gene. 2006 Oct 1;380(2):72-83. Epub 2006 Jun 3. PMID:16828986 doi:http://dx.doi.org/S0378-1119(06)00340-4
- ↑ Wang PY, Seabold GK, Wenthold RJ. Synaptic adhesion-like molecules (SALMs) promote neurite outgrowth. Mol Cell Neurosci. 2008 Sep;39(1):83-94. doi: 10.1016/j.mcn.2008.05.019. Epub, 2008 Jun 7. PMID:18585462 doi:http://dx.doi.org/10.1016/j.mcn.2008.05.019
- ↑ Ulyanova T, Blasioli J, Woodford-Thomas TA, Thomas ML. The sialoadhesin CD33 is a myeloid-specific inhibitory receptor. Eur J Immunol. 1999 Nov;29(11):3440-9. PMID:10556798 doi:http://dx.doi.org/10.1002/(SICI)1521-4141(199911)29:11<3440::AID-IMMU3440>3.0.CO;2-C
- ↑ Vitale C, Romagnani C, Puccetti A, Olive D, Costello R, Chiossone L, Pitto A, Bacigalupo A, Moretta L, Mingari MC. Surface expression and function of p75/AIRM-1 or CD33 in acute myeloid leukemias: engagement of CD33 induces apoptosis of leukemic cells. Proc Natl Acad Sci U S A. 2001 May 8;98(10):5764-9. Epub 2001 Apr 24. PMID:11320212 doi:http://dx.doi.org/10.1073/pnas.091097198
- ↑ Karki S, Shkumatov AV, Bae S, Kim H, Ko J, Kajander T. Structural basis of SALM3 dimerization and synaptic adhesion complex formation with PTPsigma. Sci Rep. 2020 Jul 14;10(1):11557. doi: 10.1038/s41598-020-68502-4. PMID:32665594 doi:http://dx.doi.org/10.1038/s41598-020-68502-4
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