1c9u

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{{STRUCTURE_1c9u| PDB=1c9u | SCENE= }}
{{STRUCTURE_1c9u| PDB=1c9u | SCENE= }}
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'''CRYSTAL STRUCTURE OF THE SOLUBLE QUINOPROTEIN GLUCOSE DEHYDROGENASE IN COMPLEX WITH PQQ'''
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===CRYSTAL STRUCTURE OF THE SOLUBLE QUINOPROTEIN GLUCOSE DEHYDROGENASE IN COMPLEX WITH PQQ===
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==Overview==
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Soluble glucose dehydrogenase (s-GDH; EC 1.1.99.17) is a classical quinoprotein which requires the cofactor pyrroloquinoline quinone (PQQ) to oxidize glucose to gluconolactone. The reaction mechanism of PQQ-dependent enzymes has remained controversial due to the absence of comprehensive structural data. We have determined the X-ray structure of s-GDH with the cofactor at 2.2 A resolution, and of a complex with reduced PQQ and glucose at 1.9 A resolution. These structures reveal the active site of s-GDH, and show for the first time how a functionally bound substrate interacts with the cofactor in a PQQ-dependent enzyme. Twenty years after the discovery of PQQ, our results finally provide conclusive evidence for a reaction mechanism comprising general base-catalyzed hydride transfer, rather than the generally accepted covalent addition-elimination mechanism. Thus, PQQ-dependent enzymes use a mechanism similar to that of nicotinamide- and flavin-dependent oxidoreductases.
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{{ABSTRACT_PUBMED_10508152}}
==About this Structure==
==About this Structure==
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[[Category: Cofactor binding]]
[[Category: Cofactor binding]]
[[Category: Superbarrel]]
[[Category: Superbarrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:30:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:25:12 2008''

Revision as of 17:25, 30 June 2008

Image:1c9u.png

Template:STRUCTURE 1c9u

CRYSTAL STRUCTURE OF THE SOLUBLE QUINOPROTEIN GLUCOSE DEHYDROGENASE IN COMPLEX WITH PQQ

Template:ABSTRACT PUBMED 10508152

About this Structure

1C9U is a Single protein structure of sequence from Acinetobacter calcoaceticus. Full crystallographic information is available from OCA.

Reference

Structure and mechanism of soluble quinoprotein glucose dehydrogenase., Oubrie A, Rozeboom HJ, Kalk KH, Olsthoorn AJ, Duine JA, Dijkstra BW, EMBO J. 1999 Oct 1;18(19):5187-94. PMID:10508152

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