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1cbk

From Proteopedia

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{{STRUCTURE_1cbk| PDB=1cbk | SCENE= }}
{{STRUCTURE_1cbk| PDB=1cbk | SCENE= }}
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'''7,8-DIHYDRO-6-HYDROXYMETHYLPTERIN-PYROPHOSPHOKINASE FROM HAEMOPHILUS INFLUENZAE'''
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===7,8-DIHYDRO-6-HYDROXYMETHYLPTERIN-PYROPHOSPHOKINASE FROM HAEMOPHILUS INFLUENZAE===
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==Overview==
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The gene encoding the 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase of Haemophilus influenzae has been cloned and expressed in Escherichia coli. A complex of the purified protein with a substrate analog has been crystallized and its structure solved by multiple anomalous dispersion using phase information obtained from a single crystal of selenomethione-labeled protein. The enzyme folds into a four-stranded antiparallel beta-sheet flanked on one side by two alpha-helices and on the other by three consecutive alpha-helices, giving a novel beta1alpha1beta2beta3alpha2beta4alpha3alpha4alpha5 polypeptide topology. The three-dimensional structure of a binary complex has been refined at 2.1 A resolution. The location of the substrate analog and a sulfate ion gives important insight into the molecular mechanism of the enzyme.
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(as it appears on PubMed at http://www.pubmed.gov), where 10080886 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10080886}}
==About this Structure==
==About this Structure==
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[[Category: Oefner, C.]]
[[Category: Oefner, C.]]
[[Category: Pyrophosphokinase]]
[[Category: Pyrophosphokinase]]
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Revision as of 17:31, 30 June 2008

Image:1cbk.png

Template:STRUCTURE 1cbk

7,8-DIHYDRO-6-HYDROXYMETHYLPTERIN-PYROPHOSPHOKINASE FROM HAEMOPHILUS INFLUENZAE

Template:ABSTRACT PUBMED 10080886

About this Structure

1CBK is a Single protein structure of sequence from Haemophilus influenzae. Full crystallographic information is available from OCA.

Reference

The structure and function of the 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase from Haemophilus influenzae., Hennig M, Dale GE, D'arcy A, Danel F, Fischer S, Gray CP, Jolidon S, Muller F, Page MG, Pattison P, Oefner C, J Mol Biol. 1999 Mar 26;287(2):211-9. PMID:10080886

Page seeded by OCA on Mon Jun 30 20:31:01 2008

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