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| | <StructureSection load='5z22' size='340' side='right'caption='[[5z22]], [[Resolution|resolution]] 1.50Å' scene=''> | | <StructureSection load='5z22' size='340' side='right'caption='[[5z22]], [[Resolution|resolution]] 1.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5z22]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cerrena Cerrena]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z22 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5Z22 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5z22]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cerrena Cerrena]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z22 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z22 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5z22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z22 OCA], [http://pdbe.org/5z22 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z22 RCSB], [http://www.ebi.ac.uk/pdbsum/5z22 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z22 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z22 OCA], [https://pdbe.org/5z22 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z22 RCSB], [https://www.ebi.ac.uk/pdbsum/5z22 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z22 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Cerrena]] | | [[Category: Cerrena]] |
| - | [[Category: Laccase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ho, T H]] | + | [[Category: Ho TH]] |
| - | [[Category: Lee, C C]] | + | [[Category: Lee CC]] |
| - | [[Category: Wang, A H.J]] | + | [[Category: Wang AHJ]] |
| - | [[Category: Wu, M H]] | + | [[Category: Wu MH]] |
| - | [[Category: Copper-dependent enzyme]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| Structural highlights
5z22 is a 1 chain structure with sequence from Cerrena. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 1.5Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Publication Abstract from PubMed
Laccases that are tolerant to organic solvents are powerful bio-catalysts with broad applications in biotechnology. Most of these uses must be accomplished at high concentration of organic solvents, during which proteins undergo unfolding, thereby losing enzyme activity. Here we show that organic-solvent pre-incubation provides effective and reversible 1.5- to 4.0-fold enhancement of enzyme activity of fungal laccases. Several organic solvents, including acetone, methanol, ethanol, DMSO, and DMF had an enhancement effect among all laccases studied. The enhancement was not substrate-specific and could be observed by using both phenolic and non-phenolic substrates. Laccase preincubated with organic solvents was sensitive to high temperature but remained stable at 25 degrees C, for an advantage for long-term storage. The acetone-pre-incubated 3-D structure of DLac, a high-efficiency fungal laccase, was determined and confirmed that the DLac protein structure remains intact and stable at a high concentration of organic solvent. Moreover, the turnover rates of fungal laccases were improved after organic-solvent pre-incubation, with DLac showing the highest enhancement among the fungal laccases examined. Our investigation sheds light on improving fungal laccase usage under extreme conditions and extends opportunities for bioremediation, decolorization, and organic synthesis.
Enhancement of laccase activity by pre-incubation with organic solvents.,Wu MH, Lin MC, Lee CC, Yu SM, Wang AH, Ho TD Sci Rep. 2019 Jul 5;9(1):9754. doi: 10.1038/s41598-019-45118-x. PMID:31278318[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Wu MH, Lin MC, Lee CC, Yu SM, Wang AH, Ho TD. Enhancement of laccase activity by pre-incubation with organic solvents. Sci Rep. 2019 Jul 5;9(1):9754. doi: 10.1038/s41598-019-45118-x. PMID:31278318 doi:http://dx.doi.org/10.1038/s41598-019-45118-x
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