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| | <StructureSection load='6vw4' size='340' side='right'caption='[[6vw4]], [[Resolution|resolution]] 2.35Å' scene=''> | | <StructureSection load='6vw4' size='340' side='right'caption='[[6vw4]], [[Resolution|resolution]] 2.35Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6vw4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Catad Catad]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VW4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6VW4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6vw4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Catenulispora_acidiphila_DSM_44928 Catenulispora acidiphila DSM 44928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VW4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VW4 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SDN:[(3S)-9-HYDROXY-1-METHYL-10-OXO-4,10-DIHYDRO-3H-BENZO[G]ISOCHROMEN-3-YL]ACETIC+ACID'>SDN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6p77|6p77]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SDN:[(3S)-9-HYDROXY-1-METHYL-10-OXO-4,10-DIHYDRO-3H-BENZO[G]ISOCHROMEN-3-YL]ACETIC+ACID'>SDN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Caci_6494 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=479433 CATAD])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vw4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vw4 OCA], [https://pdbe.org/6vw4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vw4 RCSB], [https://www.ebi.ac.uk/pdbsum/6vw4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vw4 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6vw4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vw4 OCA], [http://pdbe.org/6vw4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vw4 RCSB], [http://www.ebi.ac.uk/pdbsum/6vw4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vw4 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/C7PWR4_CATAD C7PWR4_CATAD] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Catad]] | + | [[Category: Catenulispora acidiphila DSM 44928]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Silvaggi, N R]] | + | [[Category: Silvaggi NR]] |
| - | [[Category: Vuksanovic, N]] | + | [[Category: Vuksanovic N]] |
| - | [[Category: Ntf2 superfamily]]
| + | |
| - | [[Category: Unknown function]]
| + | |
| Structural highlights
Function
C7PWR4_CATAD
Publication Abstract from PubMed
Proteins belonging to the NTF2-like superfamily are present in the biosynthetic pathways of numerous polyketide natural products, such as anthracyclins and benzoisochromanequinones. Some have been found to be bona fide polyketide cyclases, but many of them have roles that are currently unknown. Here, the X-ray crystal structures of three NTF2-like proteins of unknown function are reported: those of ActVI-ORFA from Streptomyces coelicolor A3(2) and its homologs Caci_6494, a protein from an uncharacterized biosynthetic cluster in Catenulispora acidiphila, and Aln2 from Streptomyces sp. CM020, a protein in the biosynthetic pathway of alnumycin. The presence of a solvent-accessible cavity and the conservation of the His/Asp dyad that is characteristic of many polyketide cyclases suggest a potential enzymatic role for these enzymes in polyketide biosynthesis.
Structural characterization of three noncanonical NTF2-like superfamily proteins: implications for polyketide biosynthesis.,Vuksanovic N, Zhu X, Serrano DA, Siitonen V, Metsa-Ketela M, Melancon CE 3rd, Silvaggi NR Acta Crystallogr F Struct Biol Commun. 2020 Aug 1;76(Pt 8):372-383. doi:, 10.1107/S2053230X20009814. Epub 2020 Jul 29. PMID:32744249[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Vuksanovic N, Zhu X, Serrano DA, Siitonen V, Metsa-Ketela M, Melancon CE 3rd, Silvaggi NR. Structural characterization of three noncanonical NTF2-like superfamily proteins: implications for polyketide biosynthesis. Acta Crystallogr F Struct Biol Commun. 2020 Aug 1;76(Pt 8):372-383. doi:, 10.1107/S2053230X20009814. Epub 2020 Jul 29. PMID:32744249 doi:http://dx.doi.org/10.1107/S2053230X20009814
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