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| | <StructureSection load='5lq5' size='340' side='right'caption='[[5lq5]], [[Resolution|resolution]] 1.46Å' scene=''> | | <StructureSection load='5lq5' size='340' side='right'caption='[[5lq5]], [[Resolution|resolution]] 1.46Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5lq5]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LQ5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LQ5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5lq5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Prochlorococcus_marinus_str._MIT_9301 Prochlorococcus marinus str. MIT 9301]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LQ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LQ5 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO3:PHOSPHITE+ION'>PO3</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.46Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5lq1|5lq1]], [[5jvb|5jvb]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO3:PHOSPHITE+ION'>PO3</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5lq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lq5 OCA], [http://pdbe.org/5lq5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lq5 RCSB], [http://www.ebi.ac.uk/pdbsum/5lq5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lq5 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lq5 OCA], [https://pdbe.org/5lq5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lq5 RCSB], [https://www.ebi.ac.uk/pdbsum/5lq5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lq5 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PHND1_PROM0 PHND1_PROM0]] Probably part of the ABC transporter complex PhnD1C1E1. Binds strongly to inorganic phosphite and with very weak affinities to methylphosphonate (MPn) and phosphate.<ref>PMID:22011717</ref> | + | [https://www.uniprot.org/uniprot/PHND1_PROM0 PHND1_PROM0] Probably part of the ABC transporter complex PhnD1C1E1. Binds strongly to inorganic phosphite and with very weak affinities to methylphosphonate (MPn) and phosphate.<ref>PMID:22011717</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Adams, N B.P]] | + | [[Category: Prochlorococcus marinus str. MIT 9301]] |
| - | [[Category: Bibby, T S]] | + | [[Category: Adams NBP]] |
| - | [[Category: Bisson, C]] | + | [[Category: Bibby TS]] |
| - | [[Category: Hitchcock, A]] | + | [[Category: Bisson C]] |
| - | [[Category: Hunter, C N]] | + | [[Category: Hitchcock A]] |
| - | [[Category: Polyviou, D]] | + | [[Category: Hunter CN]] |
| - | [[Category: Abc-transporter]]
| + | [[Category: Polyviou D]] |
| - | [[Category: Periplasmic binding protein]]
| + | |
| - | [[Category: Phosphite]]
| + | |
| - | [[Category: Prochlorococcus]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
PHND1_PROM0 Probably part of the ABC transporter complex PhnD1C1E1. Binds strongly to inorganic phosphite and with very weak affinities to methylphosphonate (MPn) and phosphate.[1]
Publication Abstract from PubMed
Inorganic phosphate is the major bioavailable form of the essential nutrient phosphorus. However, the concentration of phosphate in most natural habitats is low enough to limit microbial growth. Under phosphate-depleted conditions some bacteria utilise phosphite and hypophosphite as alternative sources of phosphorus, but the molecular basis of reduced phosphorus acquisition from the environment is not fully understood. Here, we present crystal structures and ligand binding affinities of periplasmic binding proteins from bacterial phosphite and hypophosphite ATP-binding cassette transporters. We reveal that phosphite and hypophosphite specificity results from a combination of steric selection and the presence of a P-H...pi interaction between the ligand and a conserved aromatic residue in the ligand-binding pocket. The characterisation of high affinity and specific transporters has implications for the marine phosphorus redox cycle, and might aid the use of phosphite as an alternative phosphorus source in biotechnological, industrial and agricultural applications.
The molecular basis of phosphite and hypophosphite recognition by ABC-transporters.,Bisson C, Adams NBP, Stevenson B, Brindley AA, Polyviou D, Bibby TS, Baker PJ, Hunter CN, Hitchcock A Nat Commun. 2017 Nov 23;8(1):1746. doi: 10.1038/s41467-017-01226-8. PMID:29170493[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Feingersch R, Philosof A, Mejuch T, Glaser F, Alalouf O, Shoham Y, Beja O. Potential for phosphite and phosphonate utilization by Prochlorococcus. ISME J. 2012 Apr;6(4):827-34. doi: 10.1038/ismej.2011.149. Epub 2011 Oct 20. PMID:22011717 doi:http://dx.doi.org/10.1038/ismej.2011.149
- ↑ Bisson C, Adams NBP, Stevenson B, Brindley AA, Polyviou D, Bibby TS, Baker PJ, Hunter CN, Hitchcock A. The molecular basis of phosphite and hypophosphite recognition by ABC-transporters. Nat Commun. 2017 Nov 23;8(1):1746. doi: 10.1038/s41467-017-01226-8. PMID:29170493 doi:http://dx.doi.org/10.1038/s41467-017-01226-8
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