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| - | [[Image:1cem.jpg|left|200px]] | + | {{Seed}} |
| | + | [[Image:1cem.png|left|200px]] |
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| | {{STRUCTURE_1cem| PDB=1cem | SCENE= }} | | {{STRUCTURE_1cem| PDB=1cem | SCENE= }} |
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| - | '''ENDOGLUCANASE A (CELA) CATALYTIC CORE, RESIDUES 33-395'''
| + | ===ENDOGLUCANASE A (CELA) CATALYTIC CORE, RESIDUES 33-395=== |
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| - | ==Overview==
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| - | BACKGROUND. Cellulases, which catalyze the hydrolysis of glycosidic bonds in cellulose, can be classified into several different protein families. Endoglucanase CelA is a member of glycosyl hydrolase family 8, a family for which no structural information was previously available. RESULTS. The crystal structure of CelA was determined by multiple isomorphous replacement and refined to 1.65 A resolution. The protein folds into a regular (alpha/alpha)6 barrel formed by six inner and six outer alpha helices. Cello-oligosaccharides bind to an acidic cleft containing at least five D-glucosyl-binding subsites (A-E) such that the scissile glycosidic linkage lies between subsites C and D. The strictly conserved residue Glu95, which occupies the center of the substrate-binding cleft and is hydrogen bonded to the glycosidic oxygen, has been assigned the catalytic role of proton donor. CONCLUSIONS. The present analysis provides a basis for modeling homologous family 8 cellulases. The architecture of the active-site cleft, presenting at least five glucosyl-binding subsites, explains why family 8 cellulases cleave cello-oligosaccharide polymers that are at least five D-glycosyl subunits long. Furthermore, the structure of CelA allows comparison with (alpha/alpha)6 barrel glycosidases that are not related in sequence, suggesting a possible, albeit distant, evolutionary relationship between different families of glycosyl hydrolases.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_8805535}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 8805535 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_8805535}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Glycosyl hydrolase]] | | [[Category: Glycosyl hydrolase]] |
| | [[Category: Glycosyltransferase]] | | [[Category: Glycosyltransferase]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:38:56 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:38:31 2008'' |
Revision as of 17:38, 30 June 2008
Template:STRUCTURE 1cem
ENDOGLUCANASE A (CELA) CATALYTIC CORE, RESIDUES 33-395
Template:ABSTRACT PUBMED 8805535
About this Structure
1CEM is a Single protein structure of sequence from Clostridium thermocellum. Full crystallographic information is available from OCA.
Reference
The crystal structure of endoglucanase CelA, a family 8 glycosyl hydrolase from Clostridium thermocellum., Alzari PM, Souchon H, Dominguez R, Structure. 1996 Mar 15;4(3):265-75. PMID:8805535
Page seeded by OCA on Mon Jun 30 20:38:31 2008
