6ura
From Proteopedia
(Difference between revisions)
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==Crystal structure of RUBISCO from Promineofilum breve== | ==Crystal structure of RUBISCO from Promineofilum breve== | ||
| - | <StructureSection load='6ura' size='340' side='right'caption='[[6ura]]' scene=''> | + | <StructureSection load='6ura' size='340' side='right'caption='[[6ura]], [[Resolution|resolution]] 2.17Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6URA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6URA FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6ura]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"candidatus_promineofilum_breve"_mcilroy_et_al._2016 "candidatus promineofilum breve" mcilroy et al. 2016]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6URA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6URA FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ura FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ura OCA], [http://pdbe.org/6ura PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ura RCSB], [http://www.ebi.ac.uk/pdbsum/6ura PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ura ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAP:2-CARBOXYARABINITOL-1,5-DIPHOSPHATE'>CAP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cbbL, CFX0092_B0544 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1806508 "Candidatus Promineofilum breve" McIlroy et al. 2016])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribulose-bisphosphate_carboxylase Ribulose-bisphosphate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.39 4.1.1.39] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ura FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ura OCA], [http://pdbe.org/6ura PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ura RCSB], [http://www.ebi.ac.uk/pdbsum/6ura PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ura ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Rubisco sustains the biosphere through the fixation of CO2 into biomass. In plants and cyanobacteria, form I Rubisco is structurally comprised of large and small subunits, whereas all other Rubisco forms lack small subunits. The rise of the form I complex through the innovation of small subunits represents a key, yet poorly understood, transition in Rubisco's evolution. Through metagenomic analyses, we discovered a previously uncharacterized clade sister to form I Rubisco that evolved without small subunits. This clade diverged before the evolution of cyanobacteria and the origin of the small subunit; thus, it provides a unique reference point to advance our understanding of form I Rubisco evolution. Structural and kinetic data presented here reveal how a proto-form I Rubisco assembled and functioned without the structural stability imparted from small subunits. Our findings provide insight into a key evolutionary transition of the most abundant enzyme on Earth and the predominant entry point for nearly all global organic carbon. | ||
| + | |||
| + | Novel bacterial clade reveals origin of form I Rubisco.,Banda DM, Pereira JH, Liu AK, Orr DJ, Hammel M, He C, Parry MAJ, Carmo-Silva E, Adams PD, Banfield JF, Shih PM Nat Plants. 2020 Sep;6(9):1158-1166. doi: 10.1038/s41477-020-00762-4. Epub 2020, Aug 31. PMID:32868887<ref>PMID:32868887</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6ura" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Candidatus promineofilum breve mcilroy et al. 2016]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Adams | + | [[Category: Ribulose-bisphosphate carboxylase]] |
| - | [[Category: Banda | + | [[Category: Adams, P D]] |
| - | [[Category: Liu | + | [[Category: Banda, D M]] |
| - | [[Category: Pereira | + | [[Category: Liu, A K]] |
| - | [[Category: Shih | + | [[Category: Pereira, J H]] |
| + | [[Category: Shih, P M]] | ||
| + | [[Category: Lyase]] | ||
| + | [[Category: Rubisco carboxylase]] | ||
Revision as of 10:12, 16 September 2020
Crystal structure of RUBISCO from Promineofilum breve
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