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| | <StructureSection load='5mdt' size='340' side='right'caption='[[5mdt]], [[Resolution|resolution]] 1.62Å' scene=''> | | <StructureSection load='5mdt' size='340' side='right'caption='[[5mdt]], [[Resolution|resolution]] 1.62Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5mdt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MDT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5MDT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5mdt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MDT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MDT FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">seb1, SPAC222.09 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.62Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5mdt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mdt OCA], [http://pdbe.org/5mdt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mdt RCSB], [http://www.ebi.ac.uk/pdbsum/5mdt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mdt ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mdt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mdt OCA], [https://pdbe.org/5mdt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mdt RCSB], [https://www.ebi.ac.uk/pdbsum/5mdt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mdt ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SEB1_SCHPO SEB1_SCHPO]] Involved in the processing of pol II transcripts.<ref>PMID:12907709</ref> | + | [https://www.uniprot.org/uniprot/SEB1_SCHPO SEB1_SCHPO] Involved in the processing of pol II transcripts.<ref>PMID:12907709</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Fission yeast]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Grimes, J]] | + | [[Category: Schizosaccharomyces pombe 972h-]] |
| - | [[Category: Renner, M]] | + | [[Category: Grimes J]] |
| - | [[Category: Vasiljeva, L]] | + | [[Category: Renner M]] |
| - | [[Category: Wittmann, S]] | + | [[Category: Vasiljeva L]] |
| - | [[Category: Rna polymerase ii]]
| + | [[Category: Wittmann S]] |
| - | [[Category: S. pombe]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcription termination]]
| + | |
| Structural highlights
Function
SEB1_SCHPO Involved in the processing of pol II transcripts.[1]
Publication Abstract from PubMed
Termination of RNA polymerase II (Pol II) transcription is an important step in the transcription cycle, which involves the dislodgement of polymerase from DNA, leading to release of a functional transcript. Recent studies have identified the key players required for this process and showed that a common feature of these proteins is a conserved domain that interacts with the phosphorylated C-terminus of Pol II (CTD-interacting domain, CID). However, the mechanism by which transcription termination is achieved is not understood. Using genome-wide methods, here we show that the fission yeast CID-protein Seb1 is essential for termination of protein-coding and non-coding genes through interaction with S2-phosphorylated Pol II and nascent RNA. Furthermore, we present the crystal structures of the Seb1 CTD- and RNA-binding modules. Unexpectedly, the latter reveals an intertwined two-domain arrangement of a canonical RRM and second domain. These results provide important insights into the mechanism underlying eukaryotic transcription termination.
The conserved protein Seb1 drives transcription termination by binding RNA polymerase II and nascent RNA.,Wittmann S, Renner M, Watts BR, Adams O, Huseyin M, Baejen C, El Omari K, Kilchert C, Heo DH, Kecman T, Cramer P, Grimes JM, Vasiljeva L Nat Commun. 2017 Apr 3;8:14861. doi: 10.1038/ncomms14861. PMID:28367989[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Mitsuzawa H, Kanda E, Ishihama A. Rpb7 subunit of RNA polymerase II interacts with an RNA-binding protein involved in processing of transcripts. Nucleic Acids Res. 2003 Aug 15;31(16):4696-701. PMID:12907709
- ↑ Wittmann S, Renner M, Watts BR, Adams O, Huseyin M, Baejen C, El Omari K, Kilchert C, Heo DH, Kecman T, Cramer P, Grimes JM, Vasiljeva L. The conserved protein Seb1 drives transcription termination by binding RNA polymerase II and nascent RNA. Nat Commun. 2017 Apr 3;8:14861. doi: 10.1038/ncomms14861. PMID:28367989 doi:http://dx.doi.org/10.1038/ncomms14861
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