This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1cfl

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1cfl.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1cfl.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1cfl| PDB=1cfl | SCENE= }}
{{STRUCTURE_1cfl| PDB=1cfl | SCENE= }}
-
'''DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT'''
+
===DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT===
-
==Overview==
+
<!--
-
The pyrimidine(6-4)pyrimidone photoproduct [(6-4) adduct] is one of the major photoproducts induced by UV irradiation of DNA and occurs at TpT sites. The (6-4) adduct is highly mutagenic and leads most often to a 3' T --&gt; C transition with 85% replicating error frequency [LeClerc, J. E., Borden, A. &amp; Lawrence, C. W. (1991) Proc. Natl. Acad. Sci. USA 88, 9685-9689]. To determine the origin of the specific 3' T --&gt; C transition of the (6-4) adduct, we have used experimental NMR restraints and molecular dynamics to determine the solution structure of a (6-4)-lesion DNA decamer duplex that contains a mismatched base pair between the 3' T residue and an opposed G residue. Normal Watson-Crick-type hydrogen bonding is retained at the 5' T of the lesion site. The O2 carbonyl of the 3' T residue forms hydrogen bonds with the imino and amino protons of the opposed G residue. This potential hydrogen bonding stabilizes the overall helix and restores the highly distorted conformation of the (6-4) adduct to the typical B-form-like DNA structure. This structural feature can explain the marked preference for the insertion of an A residue opposite the 5' T and a G residue opposite the 3' T of the (6-4) lesion during trans-lesion synthesis. Thus these insertions yield the predominant 3' T --&gt; C transition.
+
The line below this paragraph, {{ABSTRACT_PUBMED_10359763}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 10359763 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_10359763}}
==About this Structure==
==About this Structure==
-
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFL OCA].
+
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFL OCA].
==Reference==
==Reference==
Line 27: Line 31:
[[Category: Mutagenesis]]
[[Category: Mutagenesis]]
[[Category: Nmr]]
[[Category: Nmr]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:41:01 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:41:00 2008''

Revision as of 17:41, 30 June 2008

Image:1cfl.png

Template:STRUCTURE 1cfl

DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT

Template:ABSTRACT PUBMED 10359763

About this Structure

Full experimental information is available from OCA.

Reference

Solution structure of a DNA decamer duplex containing the stable 3' T.G base pair of the pyrimidine(6-4)pyrimidone photoproduct [(6-4) adduct]: implications for the highly specific 3' T --> C transition of the (6-4) adduct., Lee JH, Hwang GS, Choi BS, Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6632-6. PMID:10359763

Page seeded by OCA on Mon Jun 30 20:41:00 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1cfl"
Personal tools