Journal:Acta Cryst D:S2059798320010906
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<scene name='85/859606/Cv/4'>Crystal structure of CYRI-B</scene>. Cartoon representation of the native CYRI-B from ''Rhincodon typus'' showing the N-terminal (''lime''), Medial (''yellow'') and C-terminal (''royalblue'') subdomains. The myristoylation site of the protein is indicated. It reveals a protein comprised solely of α-helices which can be divided into three linear subdomains. The <scene name='85/859606/Cv/5'>N-terminal subdomain</scene> extends until residue 125 and consists of five α-helices, three of which form a 60 Å elongated α-helical hairpin extending the entire length of the protein. <scene name='85/859606/Cv/7'>The Medial subdomain</scene> extends from residues 126 to 214 and exhibits a 90º-broken antiparallel helical hairpin, that covers one face of the N-terminal subdomain. The two antiparallel helices α6 and α9 split at the lowest part to surround the extended α4 helix of the N-terminal subdomain. Finally, the <scene name='85/859606/Cv/8'>C-terminal subdomain</scene> (215-324) consists of six α-helices which associate into a globular bundle located at the base of the structure. This bundle caps the lower half of the Medial subdomain and is elevated above helices α1 and α5 of the N-terminal subdomain. In both the native and SeMet structures, the flexible loop composed of residues 171 to 175 was too disordered to build and is therefore missing from the final models. This loop connects helices α7 and α8 of the Medial subdomain. A non-native serine, residual from the purification strategy, is present at the N-terminus of the structure. | <scene name='85/859606/Cv/4'>Crystal structure of CYRI-B</scene>. Cartoon representation of the native CYRI-B from ''Rhincodon typus'' showing the N-terminal (''lime''), Medial (''yellow'') and C-terminal (''royalblue'') subdomains. The myristoylation site of the protein is indicated. It reveals a protein comprised solely of α-helices which can be divided into three linear subdomains. The <scene name='85/859606/Cv/5'>N-terminal subdomain</scene> extends until residue 125 and consists of five α-helices, three of which form a 60 Å elongated α-helical hairpin extending the entire length of the protein. <scene name='85/859606/Cv/7'>The Medial subdomain</scene> extends from residues 126 to 214 and exhibits a 90º-broken antiparallel helical hairpin, that covers one face of the N-terminal subdomain. The two antiparallel helices α6 and α9 split at the lowest part to surround the extended α4 helix of the N-terminal subdomain. Finally, the <scene name='85/859606/Cv/8'>C-terminal subdomain</scene> (215-324) consists of six α-helices which associate into a globular bundle located at the base of the structure. This bundle caps the lower half of the Medial subdomain and is elevated above helices α1 and α5 of the N-terminal subdomain. In both the native and SeMet structures, the flexible loop composed of residues 171 to 175 was too disordered to build and is therefore missing from the final models. This loop connects helices α7 and α8 of the Medial subdomain. A non-native serine, residual from the purification strategy, is present at the N-terminus of the structure. | ||
| - | <scene name='85/859606/Cv/9'>CYRI-B and CYFIP1 present a high degree of structural similarity</scene>. CYRI-B showing the N-terminal (''lime''), Medial (''yellow'') and C-terminal (''royalblue'') subdomains. CYFIP1 DUF1394 domain (PDB [[3p8c]]) showing the N-terminal (''green''), Medial (''gold'') and C-terminal (''blue'') subdomains. The presence of an extra hairpin in CYFIP1 is indicated (''darkstateblue''). | + | <scene name='85/859606/Cv/9'>CYRI-B and CYFIP1 present a high degree of structural similarity</scene>. CYRI-B showing the N-terminal (''lime''), Medial (''yellow'') and C-terminal (''royalblue'') subdomains. CYFIP1 DUF1394 domain (PDB [[3p8c]]) showing the N-terminal (''green''), Medial (''gold'') and C-terminal (''blue'') subdomains. The presence of an extra hairpin in CYFIP1 is indicated (''darkstateblue''). <scene name='85/859606/Cv/10'>Animation of this scene</scene>. |
| + | <jmol><jmolButton> | ||
| + | <script>if (_animating); anim pause;set echo bottom left; color echo white; font echo 20 sansserif;echo Animation Paused; else; anim resume; set echo off;endif;</script> | ||
| + | <text>Pause/Start Animation</text> | ||
| + | </jmolButton></jmol> | ||
<b>References</b><br> | <b>References</b><br> | ||
Revision as of 13:36, 25 August 2020
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