1ci4

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{{STRUCTURE_1ci4| PDB=1ci4 | SCENE= }}
{{STRUCTURE_1ci4| PDB=1ci4 | SCENE= }}
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'''THE CRYSTAL STRUCTURE OF HUMAN BARRIER-TO-AUTOINTEGRATION FACTOR (BAF)'''
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===THE CRYSTAL STRUCTURE OF HUMAN BARRIER-TO-AUTOINTEGRATION FACTOR (BAF)===
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==Overview==
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Barrier-to-autointegration factor (BAF) is a host cell protein that plays a crucial role in retroviral integration. Preintegration complexes (PICs) stripped of BAF lose their normal integration activity, which can be restored by incubation with purified BAF. BAF bridges double-stranded DNA both intra- and intermolecularly in a non-sequence-specific manner, leading to the formation of a nucleoprotein network. BAF also binds to the nuclear protein lamina-associated polypeptide 2 (LAP2), and is localized with chromatin during interphase and mitosis. The crystal structure of homodimeric human BAF has been determined to 1.9 A resolution. The fold of the BAF monomer resembles that of the second domain of RuvA. This comparison revealed the presence of the helix-hairpin-helix (HhH) nonspecific DNA binding motif within BAF. A novel feature of BAF's HhH motif is the occupation of the metal binding site by the epsilon-amino group of Lys 6, providing an alternative means of sequestering positive charge. Mutational analysis corroborates the HhH motif's prominent role in DNA binding and argues against a previously proposed helix-turn-helix (HTH) binding site located in another region of the monomer. A model of BAF bridging DNA via the HhH motif is proposed.
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(as it appears on PubMed at http://www.pubmed.gov), where 10924106 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10924106}}
==About this Structure==
==About this Structure==
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[[Category: Preintegration complex]]
[[Category: Preintegration complex]]
[[Category: Retroviral integration]]
[[Category: Retroviral integration]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:45:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:46:30 2008''

Revision as of 17:46, 30 June 2008

Image:1ci4.png

Template:STRUCTURE 1ci4

THE CRYSTAL STRUCTURE OF HUMAN BARRIER-TO-AUTOINTEGRATION FACTOR (BAF)

Template:ABSTRACT PUBMED 10924106

About this Structure

1CI4 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural basis of DNA bridging by barrier-to-autointegration factor., Umland TC, Wei SQ, Craigie R, Davies DR, Biochemistry. 2000 Aug 8;39(31):9130-8. PMID:10924106

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