1ci5

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[[Image:1ci5.jpg|left|200px]]
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{{Seed}}
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{{STRUCTURE_1ci5| PDB=1ci5 | SCENE= }}
{{STRUCTURE_1ci5| PDB=1ci5 | SCENE= }}
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'''GLYCAN-FREE MUTANT ADHESION DOMAIN OF HUMAN CD58 (LFA-3)'''
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===GLYCAN-FREE MUTANT ADHESION DOMAIN OF HUMAN CD58 (LFA-3)===
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==Overview==
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A general strategy is presented here for producing glycan-free forms of glycoproteins without loss of function by employing apolar-to-polar mutations of surface residues in functionally irrelevant epitopes. The success of this structure-based approach was demonstrated through the expression in Escherichia coli of a soluble 11 kDa adhesion domain extracted from the heavily glycosylated 55 kDa human CD58 ectodomain. The solution structure was subsequently determined and binding to its counter-receptor CD2 studied by NMR. This mutant adhesion domain is functional as determined by several experimental methods, and the size of its binding site has been probed by chemical shift perturbations in NMR titration experiments. The new structural information supports a 'hand-shake' model of CD2-CD58 interaction involving the GFCC'C" faces of both CD2 and CD58 adhesion domains. The region responsible for binding specificity is most likely localized on the C, C' and C" strands and the C-C' and C'-C" loops on CD58.
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(as it appears on PubMed at http://www.pubmed.gov), where 10357807 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10357807}}
==About this Structure==
==About this Structure==
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1CI5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CI5 OCA].
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1CI5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CI5 OCA].
==Reference==
==Reference==
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[[Category: Cell surface receptor]]
[[Category: Cell surface receptor]]
[[Category: Immunoglobulin superfamily v-set domain]]
[[Category: Immunoglobulin superfamily v-set domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:45:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:46:27 2008''

Revision as of 17:46, 30 June 2008

Image:1ci5.png

Template:STRUCTURE 1ci5

GLYCAN-FREE MUTANT ADHESION DOMAIN OF HUMAN CD58 (LFA-3)

Template:ABSTRACT PUBMED 10357807

About this Structure

1CI5 is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Functional glycan-free adhesion domain of human cell surface receptor CD58: design, production and NMR studies., Sun ZY, Dotsch V, Kim M, Li J, Reinherz EL, Wagner G, EMBO J. 1999 Jun 1;18(11):2941-9. PMID:10357807

Page seeded by OCA on Mon Jun 30 20:46:27 2008

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