2ifs

<StructureSection load='2ifs' size='340' side='right'caption='[[2ifs]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>

<table><tr><td colspan='2'>[[2ifs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IFS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2IFS FirstGlance]. <br>

</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">WASPIP, wasl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2ifs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ifs OCA], [http://pdbe.org/2ifs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ifs RCSB], [http://www.ebi.ac.uk/pdbsum/2ifs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ifs ProSAT]</span></td></tr>

== Disease ==

[[http://www.uniprot.org/uniprot/WIPF1_HUMAN WIPF1_HUMAN]] Defects in WIPF1 are the cause of Wiskott-Aldrich syndrome type 2 (WAS2) [MIM:[http://omim.org/entry/614493 614493]]. WAS2 is an immunodeficiency disorder characterized by eczema, thrombocytopenia, recurrent infections, defective T-cell proliferation, and impaired natural killer cell function.<ref>PMID:22231303</ref>

[[http://www.uniprot.org/uniprot/WIPF1_HUMAN WIPF1_HUMAN]] May have direct activity on the actin cytoskeleton. Induces actin polymerization and redistribution. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation (By similarity). Plays an important role in the intracellular motility of vaccinia virus by functioning as an adapter for recruiting WASL to vaccinia virus.<ref>PMID:9405671</ref> <ref>PMID:10878810</ref>

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== Publication Abstract from PubMed ==

The WASP-interacting protein (WIP) targets WASP/WAVE proteins through a constitutive interaction with an amino-terminal enabled/VASP homology (EVH1) domain. Parallel investigations had previously identified two distinct N-WASP binding motifs corresponding to WIP residues 451-461 and 461-485, and we determined the structure of a complex between WIP-(461-485) and the N-WASP EVH1 domain (Volkman, B. F., Prehoda, K. E., Scott, J. A., Peterson, F. C., and Lim, W. A. (2002) Cell 111, 565-576). The present results show that, when combined, the WIP-(451-485) sequence wraps further around the EVH1 domain, extending the interface observed previously. Specific contacts with three WIP epitopes corresponded to regions of high sequence conservation in the verprolin family. A central polyproline motif occupied the canonical binding site but in a reversed orientation relative to other EVH1 complexes. This interaction was augmented in the amino- and carboxyl-terminal directions by additional hydrophobic contacts involving WIP residues 454-459 and 475-478, respectively. Disruption of any of the three WIP epitopes reduced N-WASP binding in cells, demonstrating a functional requirement for the entire binding domain, which is significantly longer than the polyproline motifs recognized by other EVH1 domains.

Multiple WASP-interacting protein recognition motifs are required for a functional interaction with N-WASP.,Peterson FC, Deng Q, Zettl M, Prehoda KE, Lim WA, Way M, Volkman BF J Biol Chem. 2007 Mar 16;282(11):8446-53. Epub 2007 Jan 16. PMID:17229736<ref>PMID:17229736</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 2ifs" style="background-color:#fffaf0;"></div>

*[[Wiskott-Aldrich syndrome protein|Wiskott-Aldrich syndrome protein]]

[[Category: Human]]

[[Category: Deng, Q]]

[[Category: Peterson, F C]]

[[Category: Volkman, B F]]

[[Category: Polyproline]]

[[Category: Wiskott-aldrich syndrome]]