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1cj2

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{{STRUCTURE_1cj2| PDB=1cj2 | SCENE= }}
{{STRUCTURE_1cj2| PDB=1cj2 | SCENE= }}
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'''MUTANT GLN34ARG OF PARA-HYDROXYBENZOATE HYDROXYLASE'''
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===MUTANT GLN34ARG OF PARA-HYDROXYBENZOATE HYDROXYLASE===
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==Overview==
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p-Hydroxybenzoate hydroxylase (PHBH) is the archetype of the family of NAD(P)H-dependent flavoprotein aromatic hydroxylases. These enzymes share a conserved FAD-binding domain but lack a recognizable fold for binding the pyridine nucleotide. We have switched the coenzyme specificity of strictly NADPH-dependent PHBH from Pseudomonas fluorescens by site-directed mutagenesis. To that end, we altered the solvent exposed helix H2 region (residues 33-40) of the FAD-binding domain. Non-conservative selective replacements of Arg33 and Tyr38 weakened the binding of NADPH without disturbing the protein architecture. Introduction of a basic residue at position 34 increased the NADPH binding strength. Double (M2) and quadruple (M4) substitutions in the N-terminal part of helix H2 did not change the coenzyme specificity. By extending the replacements towards residues 38 and 40, M5 and M6 mutants were generated which were catalytically more efficient with NADH than with NADPH. It is concluded that specificity in P. fluorescens PHBH is conferred by interactions of Arg33, Tyr38 and Arg42 with the 2'-phosphate moiety of bound NADPH, and that introduction of an acidic group at position 38 potentially enables the recognition of the 2'-hydroxy group of NADH. This is the first report on the coenzyme reversion of a flavoprotein aromatic hydroxylase.
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(as it appears on PubMed at http://www.pubmed.gov), where 10493859 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10493859}}
==About this Structure==
==About this Structure==
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[[Category: Hydroxybenzoate]]
[[Category: Hydroxybenzoate]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:47:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:49:01 2008''

Revision as of 17:49, 30 June 2008

Image:1cj2.png

Template:STRUCTURE 1cj2

MUTANT GLN34ARG OF PARA-HYDROXYBENZOATE HYDROXYLASE

Template:ABSTRACT PUBMED 10493859

About this Structure

1CJ2 is a Single protein structure of sequence from Pseudomonas fluorescens. Full crystallographic information is available from OCA.

Reference

Switch of coenzyme specificity of p-hydroxybenzoate hydroxylase., Eppink MH, Overkamp KM, Schreuder HA, Van Berkel WJ, J Mol Biol. 1999 Sep 10;292(1):87-96. PMID:10493859

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