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6l3u
From Proteopedia
(Difference between revisions)
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==Human Cx31.3/GJC3 connexin hemichannel in the presence of calcium== | ==Human Cx31.3/GJC3 connexin hemichannel in the presence of calcium== | ||
| - | <StructureSection load='6l3u' size='340' side='right'caption='[[6l3u]]' scene=''> | + | <StructureSection load='6l3u' size='340' side='right'caption='[[6l3u]], [[Resolution|resolution]] 2.53Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L3U OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6L3U FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6l3u]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L3U OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6L3U FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6l3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l3u OCA], [http://pdbe.org/6l3u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l3u RCSB], [http://www.ebi.ac.uk/pdbsum/6l3u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l3u ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=LMN:LAURYL+MALTOSE+NEOPENTYL+GLYCOL'>LMN</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GJC3, GJE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6l3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l3u OCA], [http://pdbe.org/6l3u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l3u RCSB], [http://www.ebi.ac.uk/pdbsum/6l3u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l3u ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/CXG3_HUMAN CXG3_HUMAN]] One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Connexin family proteins assemble into hexameric channels called hemichannels/connexons, which function as transmembrane channels or dock together to form gap junction intercellular channels (GJIChs). We determined the cryo-electron microscopy structures of human connexin 31.3 (Cx31.3)/GJC3 hemichannels in the presence and absence of calcium ions and with a hearing-loss mutation R15G at 2.3-, 2.5-, and 2.6-A resolutions, respectively. Compared with available structures of GJICh in open conformation, Cx31.3 hemichannel shows substantial structural changes of highly conserved regions in the connexin family, including opening of calcium ion-binding tunnels, reorganization of salt-bridge networks, exposure of lipid-binding sites, and collocation of amino-terminal helices at the cytoplasmic entrance. We also found that the hemichannel has a pore with a diameter of ~8 A and selectively transports chloride ions. Our study provides structural insights into the permeant selectivity of Cx31.3 hemichannel. | ||
| + | |||
| + | Cryo-EM structure of human Cx31.3/GJC3 connexin hemichannel.,Lee HJ, Jeong H, Hyun J, Ryu B, Park K, Lim HH, Yoo J, Woo JS Sci Adv. 2020 Aug 28;6(35):eaba4996. doi: 10.1126/sciadv.aba4996. eCollection, 2020 Aug. PMID:32923625<ref>PMID:32923625</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6l3u" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Human]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Hyun J]] | + | [[Category: Hyun, J]] |
| - | [[Category: Jeong H]] | + | [[Category: Jeong, H]] |
| - | [[Category: Lee | + | [[Category: Lee, H J]] |
| - | [[Category: Ryu B]] | + | [[Category: Ryu, B]] |
| - | [[Category: Woo | + | [[Category: Woo, J S]] |
| + | [[Category: Atp release]] | ||
| + | [[Category: Gap junction]] | ||
| + | [[Category: Hemichannel]] | ||
| + | [[Category: Hexamer]] | ||
| + | [[Category: Membrane protein]] | ||
Current revision
Human Cx31.3/GJC3 connexin hemichannel in the presence of calcium
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Categories: Human | Large Structures | Hyun, J | Jeong, H | Lee, H J | Ryu, B | Woo, J S | Atp release | Gap junction | Hemichannel | Hexamer | Membrane protein
