6wdg

==Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure VI-B)==

<StructureSection load='6wdg' size='340' side='right'caption='[[6wdg]], [[Resolution|resolution]] 3.30&Aring;' scene=''>

<table><tr><td colspan='2'>[[6wdg]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WDG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WDG FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wdg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wdg OCA], [https://pdbe.org/6wdg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wdg RCSB], [https://www.ebi.ac.uk/pdbsum/6wdg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wdg ProSAT]</span></td></tr>

== Function ==

[https://www.uniprot.org/uniprot/RL2_ECOLI RL2_ECOLI] One of the primary rRNA binding proteins. Located near the base of the L1 stalk, it is probably also mobile. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is highly controversial.[HAMAP-Rule:MF_01320_B] In the E.coli 70S ribosome in the initiation state it has been modeled to make several contacts with the 16S rRNA (forming bridge B7b, PubMed:12809609); these contacts are broken in the model with bound EF-G.[HAMAP-Rule:MF_01320_B]

==See Also==

*[[Ribosome 3D structures|Ribosome 3D structures]]

[[Category: Escherichia coli K-12]]

[[Category: Demo G]]

[[Category: Loveland AB]]