1ckv

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[[Image:1ckv.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1ckv| PDB=1ckv | SCENE= }}
{{STRUCTURE_1ckv| PDB=1ckv | SCENE= }}
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'''STRUCTURE OF THE SOLUBLE METHANE MONOOXYGENASE REGULATORY PROTEIN B'''
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===STRUCTURE OF THE SOLUBLE METHANE MONOOXYGENASE REGULATORY PROTEIN B===
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==Overview==
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The soluble methane monooxygenase (sMMO; EC 1.14.13.25) from the pseudothermophile Methylococcus capsulatus (Bath) is a three-component enzyme system that catalyzes the selective oxidation of methane to methanol. We have used NMR spectroscopy to produce a highly refined structure of MMOB, the 16-kDa regulatory protein of this system. This structure has a unique and intricate fold containing seven beta-strands forming two beta-sheets oriented perpendicular to each other and bridged by three alpha-helices. The rate and efficiency of the methane hydroxylation by sMMO depend on dynamic binding interactions of the hydroxylase with the reductase and regulatory protein components during catalysis. We have monitored by NMR the binding of MMOB to the hydroxylase in the presence and absence of the reductase. The results of these studies provide structural insight into how the regulatory protein interacts with the hydroxylase.
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{{ABSTRACT_PUBMED_10393915}}
==About this Structure==
==About this Structure==
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1CKV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CKV OCA].
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1CKV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CKV OCA].
==Reference==
==Reference==
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[[Category: Walters, K J.]]
[[Category: Walters, K J.]]
[[Category: Hydroxylase regulatory protein]]
[[Category: Hydroxylase regulatory protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:53:21 2008''

Revision as of 17:53, 30 June 2008

Image:1ckv.png

Template:STRUCTURE 1ckv

STRUCTURE OF THE SOLUBLE METHANE MONOOXYGENASE REGULATORY PROTEIN B

Template:ABSTRACT PUBMED 10393915

About this Structure

1CKV is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

Structure of the soluble methane monooxygenase regulatory protein B., Walters KJ, Gassner GT, Lippard SJ, Wagner G, Proc Natl Acad Sci U S A. 1999 Jul 6;96(14):7877-82. PMID:10393915

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