3eyy

From Proteopedia

(Difference between revisions)

Current revision

Structural basis for the specialization of Nur, a nickel-specific Fur homologue, in metal sensing and DNA recognition

Structural highlights

3eyy is a 2 chain structure with sequence from Streptomyces coelicolor. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.4Å
Ligands:	, , , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9K4F8_STRCO

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Nur, a member of the Fur family, is a nickel-responsive transcription factor that controls nickel homeostasis and anti-oxidative response in Streptomyces coelicolor. Here we report the 2.4-A resolution crystal structure of Nur. It contains a unique nickel-specific metal site in addition to a nonspecific common metal site. The identification of the 6-5-6 motif of the Nur recognition box and a Nur/DNA complex model reveals that Nur mainly interacts with terminal bases of the palindrome on complex formation. This contrasts with more distributed contacts between Fur and the n-1-n type of the Fur-binding motif. The disparity between Nur and Fur in the conformation of the S1-S2 sheet in the DNA-binding domain can explain their different DNA-recognition patterns. Furthermore, the fact that the specificity of Nur in metal sensing and DNA recognition is conferred by the specific metal site suggests that its introduction drives the evolution of Nur orthologs in the Fur family.

Structural basis for the specialization of Nur, a nickel-specific Fur homolog, in metal sensing and DNA recognition.,An YJ, Ahn BE, Han AR, Kim HM, Chung KM, Shin JH, Cho YB, Roe JH, Cha SS Nucleic Acids Res. 2009 Jun;37(10):3442-51. Epub 2009 Mar 31. PMID:19336416^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ An YJ, Ahn BE, Han AR, Kim HM, Chung KM, Shin JH, Cho YB, Roe JH, Cha SS. Structural basis for the specialization of Nur, a nickel-specific Fur homolog, in metal sensing and DNA recognition. Nucleic Acids Res. 2009 Jun;37(10):3442-51. Epub 2009 Mar 31. PMID:19336416 doi:10.1093/nar/gkp198

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3eyy"

Categories: Large Structures | Streptomyces coelicolor | An YJ | Cha S-S

@@ Line 3: / Line 3: @@
 <StructureSection load='3eyy' size='340' side='right'caption='[[3eyy]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3eyy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces coelicolor" (muller 1908) lieske 1921]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EYY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3EYY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3eyy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EYY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EYY FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3eyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eyy OCA], [http://pdbe.org/3eyy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3eyy RCSB], [http://www.ebi.ac.uk/pdbsum/3eyy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3eyy ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eyy OCA], [https://pdbe.org/3eyy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eyy RCSB], [https://www.ebi.ac.uk/pdbsum/3eyy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eyy ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9K4F8_STRCO Q9K4F8_STRCO]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 31: / Line 34: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: An, Y J]]
+[[Category: Streptomyces coelicolor]]
-[[Category: Cha, S S]]
+[[Category: An YJ]]
-[[Category: D-domain]]
+[[Category: Cha S-S]]
-[[Category: Db-domain]]
-[[Category: Dimerization domain]]
-[[Category: Dna-binding domain]]
-[[Category: Nickel-uptake regulator]]
-[[Category: Nur]]
-[[Category: Transport]]

3eyy

From Proteopedia

Current revision

Structural basis for the specialization of Nur, a nickel-specific Fur homologue, in metal sensing and DNA recognition

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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