This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
6zxu
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Catabolic reductive dehalogenase NpRdhA, N-terminally tagged.== | ==Catabolic reductive dehalogenase NpRdhA, N-terminally tagged.== | ||
| - | <StructureSection load='6zxu' size='340' side='right'caption='[[6zxu]]' scene=''> | + | <StructureSection load='6zxu' size='340' side='right'caption='[[6zxu]], [[Resolution|resolution]] 1.73Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZXU OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6zxu]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Nitratireductor_pacificus_pht-3B Nitratireductor pacificus pht-3B]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZXU FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.73Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zxu OCA], [https://pdbe.org/6zxu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zxu RCSB], [https://www.ebi.ac.uk/pdbsum/6zxu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zxu ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/K2MB66_9HYPH K2MB66_9HYPH] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Reductive dehalogenases are responsible for the reductive cleavage of carbon-halogen bonds during organohalide respiration. A variety of mechanisms have been proposed for these cobalamin and [4Fe-4S] containing enzymes, including organocobalt, radical, or cobalt-halide adduct based catalysis. The latter was proposed for the oxygen-tolerant Nitratireductor pacificus pht-3B catabolic reductive dehalogenase (NpRdhA). Here, we present the first substrate bound NpRdhA crystal structures, confirming a direct cobalt-halogen interaction is established and providing a rationale for substrate preference. Product formation is observed in crystallo due to X-ray photoreduction. Protein engineering enables rational alteration of substrate preference, providing a future blue print for the application of this and related enzymes in bioremediation. | ||
| + | |||
| + | Catabolic Reductive Dehalogenase Substrate Complex Structures Underpin Rational Repurposing of Substrate Scope.,Halliwell T, Fisher K, Payne KAP, Rigby SEJ, Leys D Microorganisms. 2020 Sep 2;8(9). pii: microorganisms8091344. doi:, 10.3390/microorganisms8091344. PMID:32887524<ref>PMID:32887524</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6zxu" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Nitratireductor pacificus pht-3B]] | ||
[[Category: Halliwell T]] | [[Category: Halliwell T]] | ||
[[Category: Leys D]] | [[Category: Leys D]] | ||
Current revision
Catabolic reductive dehalogenase NpRdhA, N-terminally tagged.
| |||||||||||
