6z1e
From Proteopedia
(Difference between revisions)
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==Crystal structure of the AAA domain of Rubisco Activase from Nostoc sp. (strain PCC 7120)== | ==Crystal structure of the AAA domain of Rubisco Activase from Nostoc sp. (strain PCC 7120)== | ||
| - | <StructureSection load='6z1e' size='340' side='right'caption='[[6z1e]]' scene=''> | + | <StructureSection load='6z1e' size='340' side='right'caption='[[6z1e]], [[Resolution|resolution]] 2.45Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z1E OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6z1e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7120_=_FACHB-418 Nostoc sp. PCC 7120 = FACHB-418]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z1E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z1E FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.454Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z1e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z1e OCA], [https://pdbe.org/6z1e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z1e RCSB], [https://www.ebi.ac.uk/pdbsum/6z1e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z1e ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RCA_NOSS1 RCA_NOSS1] Activation of RuBisCO (ribulose-1,5-bisohosphate carboxylase/oxygenase; EC 4.1.1.39) involves the ATP-dependent carboxylation of the epsilon-amino group of lysine leading to a carbamate structure. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Rubisco, the key enzyme of CO(2) fixation in photosynthesis, is prone to inactivation by inhibitory sugar phosphates. Inhibited Rubisco undergoes conformational repair by the hexameric AAA+ chaperone Rubisco activase (Rca) in a process that is not well understood. Here, we performed a structural and mechanistic analysis of cyanobacterial Rca, a close homolog of plant Rca. In the Rca:Rubisco complex, Rca is positioned over the Rubisco catalytic site under repair and pulls the N-terminal tail of the large Rubisco subunit (RbcL) into the hexamer pore. Simultaneous displacement of the C terminus of the adjacent RbcL opens the catalytic site for inhibitor release. An alternative interaction of Rca with Rubisco is mediated by C-terminal domains that resemble the small Rubisco subunit. These domains, together with the N-terminal AAA+ hexamer, ensure that Rca is packaged with Rubisco into carboxysomes. The cyanobacterial Rca is a dual-purpose protein with functions in Rubisco repair and carboxysome organization. | ||
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| + | Dual Functions of a Rubisco Activase in Metabolic Repair and Recruitment to Carboxysomes.,Flecken M, Wang H, Popilka L, Hartl FU, Bracher A, Hayer-Hartl M Cell. 2020 Oct 15;183(2):457-473.e20. doi: 10.1016/j.cell.2020.09.010. Epub 2020 , Sep 25. PMID:32979320<ref>PMID:32979320</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6z1e" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Nostoc sp. PCC 7120 = FACHB-418]] | ||
[[Category: Bracher A]] | [[Category: Bracher A]] | ||
[[Category: Popilka L]] | [[Category: Popilka L]] | ||
Current revision
Crystal structure of the AAA domain of Rubisco Activase from Nostoc sp. (strain PCC 7120)
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