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3i5h

From Proteopedia

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Current revision

The crystal structure of rigor like squid myosin S1 in the absence of nucleotide

Structural highlights

3i5h is a 3 chain structure with sequence from Doryteuthis pealeii and Todarodes pacificus. This structure supersedes the now removed PDB entry 2ekv. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.4Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O44934_DORPE

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Unlike processive cellular motors such as myosin V, whose structure has recently been determined in a "rigor-like" conformation, myosin II from contracting muscle filaments necessarily spends most of its time detached from actin. By using squid and sea scallop sources, however, we have now obtained similar rigor-like atomic structures for muscle myosin heads (S1). The significance of the hallmark closed actin-binding cleft in these crystal structures is supported here by actin/S1-binding studies. These structures reveal how different duty ratios, and hence cellular functions, of the myosin isoforms may be accounted for, in part, on the basis of detailed differences in interdomain contacts. Moreover, the rigor-like position of switch II turns out to be unique for myosin V. The overall arrangements of subdomains in the motor are relatively conserved in each of the known contractile states, and we explore qualitatively the energetics of these states.

Rigor-like structures from muscle myosins reveal key mechanical elements in the transduction pathways of this allosteric motor.,Yang Y, Gourinath S, Kovacs M, Nyitray L, Reutzel R, Himmel DM, O'Neall-Hennessey E, Reshetnikova L, Szent-Gyorgyi AG, Brown JH, Cohen C Structure. 2007 May;15(5):553-64. PMID:17502101^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yang Y, Gourinath S, Kovacs M, Nyitray L, Reutzel R, Himmel DM, O'Neall-Hennessey E, Reshetnikova L, Szent-Gyorgyi AG, Brown JH, Cohen C. Rigor-like structures from muscle myosins reveal key mechanical elements in the transduction pathways of this allosteric motor. Structure. 2007 May;15(5):553-64. PMID:17502101 doi:10.1016/j.str.2007.03.010

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3i5h"

@@ Line 3: / Line 3: @@
 <StructureSection load='3i5h' size='340' side='right'caption='[[3i5h]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3i5h]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Loligo_pealei Loligo pealei] and [http://en.wikipedia.org/wiki/Todarodes_pacificus Todarodes pacificus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2ekv 2ekv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I5H OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3I5H FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3i5h]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Doryteuthis_pealeii Doryteuthis pealeii] and [https://en.wikipedia.org/wiki/Todarodes_pacificus Todarodes pacificus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2ekv 2ekv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I5H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I5H FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ekv|2ekv]], [[3i5f|3i5f]], [[3i5g|3i5g]], [[3i5i|3i5i]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3i5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i5h OCA], [http://pdbe.org/3i5h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3i5h RCSB], [http://www.ebi.ac.uk/pdbsum/3i5h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3i5h ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i5h OCA], [https://pdbe.org/3i5h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i5h RCSB], [https://www.ebi.ac.uk/pdbsum/3i5h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i5h ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/MLE_TODPA MLE_TODPA]] In molluscan muscle, calcium regulation is associated with myosin rather than with actin. Muscle myosin contains two types of light chains: the catalytic light chain, essential for ATPase activity, and the regulatory light chain, a calcium-binding protein responsible for Ca(2+) dependent binding and Ca(2+) dependent Mg-ATPase activity. [[http://www.uniprot.org/uniprot/MLR_TODPA MLR_TODPA]] In molluscan muscle, calcium regulation is associated with myosin rather than with actin. Muscle myosin contains two types of light chains: the catalytic light chain, essential for ATPase activity, and the regulatory light chain, a calcium-binding protein responsible for Ca(2+) dependent binding and Ca(2+) dependent Mg-ATPase activity.
+[https://www.uniprot.org/uniprot/O44934_DORPE O44934_DORPE]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 36: / Line 36: @@
 __TOC__
 </StructureSection>
+[[Category: Doryteuthis pealeii]]
 [[Category: Large Structures]]
-[[Category: Loligo pealei]]
 [[Category: Todarodes pacificus]]
-[[Category: Brown, J H]]
+[[Category: Brown JH]]
-[[Category: Cohen, C]]
+[[Category: Cohen C]]
-[[Category: Gourinath, S]]
+[[Category: Gourinath S]]
-[[Category: Himmel, D M]]
+[[Category: Himmel DM]]
-[[Category: Kovacs, M]]
+[[Category: Kovacs M]]
-[[Category: Neall-Hennessey, E O]]
+[[Category: Nyitray L]]
-[[Category: Nyitray, L]]
+[[Category: O'Neall-Hennessey E]]
-[[Category: Reshetnikova, L]]
+[[Category: Reshetnikova L]]
-[[Category: Reutzel, R]]
+[[Category: Reutzel R]]
-[[Category: Szent-Gyorgyi, A G]]
+[[Category: Szent-Gyorgyi AG]]
-[[Category: Yang, Y]]
+[[Category: Yang Y]]
-[[Category: Contractile protein]]
-[[Category: Myosin s1]]
-[[Category: Nucleotide free]]
-[[Category: Rigor-like]]
-[[Category: Squid]]

3i5h

From Proteopedia

Current revision

The crystal structure of rigor like squid myosin S1 in the absence of nucleotide

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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