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1cov

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{{STRUCTURE_1cov| PDB=1cov | SCENE= }}
{{STRUCTURE_1cov| PDB=1cov | SCENE= }}
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'''COXSACKIEVIRUS B3 COAT PROTEIN'''
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===COXSACKIEVIRUS B3 COAT PROTEIN===
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==Overview==
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The crystal structure of coxsackievirus B3 (CVB3) has been determined to 3.5 A resolution. The icosahedral CVB3 particles crystallize in the monoclinic space group, P2(1), (a = 574.6, b = 302.1, c = 521.6 A, beta = 107.7 degrees ) with two virions in the asymmetric unit giving 120-fold non-crystallographic redundancy. The crystals diffracted to 2.7 A resolution and the X-ray data set was 55% complete to 3.0,4, resolution. Systematically weak reflections and the self-rotation function established pseudo R32 symmetry with each particle sitting on a 32 special position. This constrained the orientation and position of each particle in the monoclinic cell to near face-centered positions and allowed for a total of six possible monoclinic space-group settings. Correct interpretation of the high-resolution (3.0-3.2 A) self-rotation function was instrumental in determining the deviations from R32 orientations of the virus particles in the unit cell. Accurate particle orientations permitted the correct assignment of the crystal space-group setting amongst the six ambiguous possibilities and for the correct determination of particle positions. Real-space electron-density averaging and phase refinement, using human rhinovius 14 (HRV14) as an initial phasing model, have been carried out to 3.5 A resolution. The initial structural model has been built and refined to 3.5 A resolution using X-PLOR.
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{{ABSTRACT_PUBMED_15299757}}
==About this Structure==
==About this Structure==
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[[Category: Coxsackievirus b3]]
[[Category: Coxsackievirus b3]]
[[Category: Icosahedral virus]]
[[Category: Icosahedral virus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 21:02:18 2008''

Revision as of 18:02, 30 June 2008

Image:1cov.png

Template:STRUCTURE 1cov

COXSACKIEVIRUS B3 COAT PROTEIN

Template:ABSTRACT PUBMED 15299757

About this Structure

1COV is a Single protein structure of sequence from Human coxsackievirus b3. Full crystallographic information is available from OCA.

Reference

Structure determination of coxsackievirus B3 to 3.5 A resolution., Muckelbauer JK, Kremer M, Minor I, Tong L, Zlotnick A, Johnson JE, Rossmann MG, Acta Crystallogr D Biol Crystallogr. 1995 Nov 1;51(Pt 6):871-87. PMID:15299757

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