User:Jeremiah C Hagler/SARS-CoV 2 Spike ACE2

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3. What color is the ACE2 receptor protein?
3. What color is the ACE2 receptor protein?
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Let's focus in on one of the spike subunits. I've setup a di
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If you look carefully, you'll notice that the receptor-binding domain (RBD) of one of the three subunits (green) of the spike protein has projected out to interact with ACE2 molecule. This is a conformational change that the spike protein actually makes as it makes contact with the ACE2 receptor, and requires the action of a host-cell protease (an enzyme that cuts peptide bonds in proteins), known as furin, which cuts one of the spike protein subunits. Once cut, the RBD can rearrange into the projected-form, which allows a tight interaction between the spike protein and the ACE2 receptor. This conformational change can be seen in animated form to the right:
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<Structure load='' size='350' frame='true' align='right' caption='Insert caption here' scene='85/857125/Morph_alpha_carbons_only/7' />
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Let's focus in on the projected RBD domain. Click on the green link for a close up view of that RBD in isolation.
<scene name='86/862235/Sar-cov-2_spike-ace2_rdb/1'>Isolated RDB</scene>
<scene name='86/862235/Sar-cov-2_spike-ace2_rdb/1'>Isolated RDB</scene>
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2. What color is used for the beta sheet?
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4. In this representation, what color is used for the alpha helices?
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5. How many alpha helices are there in this structure?
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6. What color is used for beta sheets?
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<scene name='86/862235/Spike-ace2_receptor_interface/1'>Spike-ACE2 interaction interface</scene>
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Now, let's add the ACE2 receptor to the scene. Click on the <scene name='86/862235/Spike-ace2_receptor_interface/1'>Spike-ACE2 interaction interface</scene> link to see the RBD interaction with the ACE2 protein.
3. Which subunit(s) (in the 3D model) is/are the MHC molecule? Which subunit(s) is/are the peptide? Think of the hot dog and bun analogy...
3. Which subunit(s) (in the 3D model) is/are the MHC molecule? Which subunit(s) is/are the peptide? Think of the hot dog and bun analogy...
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Revision as of 16:04, 23 September 2020

Protein 1: SARS-CoV-2 Spike Proteinb/ACE2 Receptor Complex

Spike/ACE2 Complex

Drag the structure with the mouse to rotate

References

Proteopedia Page Contributors and Editors (what is this?)

Jeremiah C Hagler

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