This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1cp2

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1cp2.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1cp2.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1cp2| PDB=1cp2 | SCENE= }}
{{STRUCTURE_1cp2| PDB=1cp2 | SCENE= }}
-
'''NITROGENASE IRON PROTEIN FROM CLOSTRIDIUM PASTEURIANUM'''
+
===NITROGENASE IRON PROTEIN FROM CLOSTRIDIUM PASTEURIANUM===
-
==Overview==
+
<!--
-
The nitrogenase iron (Fe) protein performs multiple functions during biological nitrogen fixation, including mediating the mechanistically essential coupling between ATP hydrolysis and electron transfer to the nitrogenase molybdenum iron (MoFe) protein during substrate reduction, and participating in the biosynthesis and insertion of the FeMo-cofactor into the MoFe-protein. To establish a structural framework for addressing the diverse functions of Fe-protein, crystal structures of the Fe-proteins from Azotobacter vinelandii and Clostridium pasteurianum have been determined at resolutions of 2.2 A and 1.93 A, respectively. These two Fe-proteins are among the more diverse in terms of amino acid sequence and biochemical properties. As described initially for the A. vinelandii Fe-protein in a different crystal form at 2.9 A resolution, each subunit of the dimeric Fe-protein adopts a polypeptide fold related to other mononucleotide-binding proteins such as G-proteins, with the two subunits bridged by a 4Fe:4S cluster. The overall similarities in the subunit fold and dimer arrangement observed in the structures of the A. vinelandii and C. pasteurianum Fe-proteins indicate that they are representative of the conformation of free Fe-protein that is not in complex with nucleotide or the MoFe-protein. Residues in the cluster and nucleotide-binding sites are linked by a network of conserved hydrogen bonds, salt-bridges and water molecules that may conformationally couple these regions. Significant variability is observed in localized regions, especially near the 4Fe:4S cluster and the MoFe-protein binding surface, that change conformation upon formation of the ADP.AlF4- stabilized complex with the MoFe-protein. A core of 140 conserved residues is identified in an alignment of 59 Fe-protein sequences that may be useful for the identification of homologous proteins with functions comparable to that of Fe-protein in non-nitrogen fixing systems.
+
The line below this paragraph, {{ABSTRACT_PUBMED_9677296}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 9677296 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_9677296}}
==About this Structure==
==About this Structure==
Line 30: Line 34:
[[Category: Nitrogenase iron protein]]
[[Category: Nitrogenase iron protein]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:57:52 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 21:02:44 2008''

Revision as of 18:02, 30 June 2008

Image:1cp2.png

Template:STRUCTURE 1cp2

NITROGENASE IRON PROTEIN FROM CLOSTRIDIUM PASTEURIANUM

Template:ABSTRACT PUBMED 9677296

About this Structure

1CP2 is a Single protein structure of sequence from Clostridium pasteurianum. Full crystallographic information is available from OCA.

Reference

Conformational variability in structures of the nitrogenase iron proteins from Azotobacter vinelandii and Clostridium pasteurianum., Schlessman JL, Woo D, Joshua-Tor L, Howard JB, Rees DC, J Mol Biol. 1998 Jul 24;280(4):669-85. PMID:9677296

Page seeded by OCA on Mon Jun 30 21:02:44 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1cp2"
Personal tools