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6swu
From Proteopedia
(Difference between revisions)
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==Crystal structure of the TPR domain of KLC1 in complex with an engineered high-affinity cargo peptide.== | ==Crystal structure of the TPR domain of KLC1 in complex with an engineered high-affinity cargo peptide.== | ||
| - | <StructureSection load='6swu' size='340' side='right'caption='[[6swu]]' scene=''> | + | <StructureSection load='6swu' size='340' side='right'caption='[[6swu]], [[Resolution|resolution]] 2.85Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SWU OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6swu]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SWU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SWU FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Klc1, Kns2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6swu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6swu OCA], [https://pdbe.org/6swu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6swu RCSB], [https://www.ebi.ac.uk/pdbsum/6swu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6swu ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Synthetic peptides are attractive candidates to manipulate protein-protein interactions inside the cell as they mimic natural interactions to compete for binding. However, protein-peptide interactions are often dynamic and weak. A challenge is to design peptides that make improved interactions with the target. Here, we devise a fragment-linking strategy-"mash-up" design-to deliver a high-affinity ligand, KinTag, for the kinesin-1 motor. Using structural insights from natural micromolar-affinity cargo-adaptor ligands, we have identified and combined key binding features in a single, high-affinity ligand. An X-ray crystal structure demonstrates interactions as designed and reveals only a modest increase in interface area. Moreover, when genetically encoded, KinTag promotes transport of lysosomes with higher efficiency than natural sequences, revealing a direct link between motor-adaptor binding affinity and organelle transport. Together, these data demonstrate a fragment-linking strategy for peptide design and its application in a synthetic motor ligand to direct cellular cargo transport. | ||
| + | |||
| + | Fragment-linking peptide design yields a high-affinity ligand for microtubule-based transport.,Cross JA, Chegkazi MS, Steiner RA, Woolfson DN, Dodding MP Cell Chem Biol. 2021 Mar 31. pii: S2451-9456(21)00149-5. doi:, 10.1016/j.chembiol.2021.03.010. PMID:33838110<ref>PMID:33838110</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6swu" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Chegkazi | + | [[Category: Lk3 transgenic mice]] |
| - | [[Category: Steiner | + | [[Category: Chegkazi, M S]] |
| + | [[Category: Steiner, R A]] | ||
| + | [[Category: Cargo recognition]] | ||
| + | [[Category: Motor protein]] | ||
| + | [[Category: Protein complex]] | ||
| + | [[Category: Protein engineering]] | ||
Revision as of 06:14, 18 August 2021
Crystal structure of the TPR domain of KLC1 in complex with an engineered high-affinity cargo peptide.
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