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6tgj
From Proteopedia
(Difference between revisions)
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==RNA-binding domain of RNase M5== | ==RNA-binding domain of RNase M5== | ||
| - | <StructureSection load='6tgj' size='340' side='right'caption='[[6tgj]]' scene=''> | + | <StructureSection load='6tgj' size='340' side='right'caption='[[6tgj]], [[Resolution|resolution]] 1.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TGJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TGJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6tgj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_12980 Atcc 12980]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TGJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TGJ FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tgj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tgj OCA], [http://pdbe.org/6tgj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tgj RCSB], [http://www.ebi.ac.uk/pdbsum/6tgj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tgj ProSAT]</span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rnmV_1, rnmV, AVP43_02013 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 ATCC 12980])</td></tr> |
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_M5 Ribonuclease M5], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.8 3.1.26.8] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tgj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tgj OCA], [http://pdbe.org/6tgj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tgj RCSB], [http://www.ebi.ac.uk/pdbsum/6tgj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tgj ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The pathways for ribosomal RNA (rRNA) maturation diverge greatly among the domains of life. In the Gram-positive model bacterium, Bacillus subtilis, the final maturation steps of the two large ribosomal subunit (50S) rRNAs, 23S and 5S pre-rRNAs, are catalyzed by the double-strand specific ribonucleases (RNases) Mini-RNase III and RNase M5, respectively. Here we present a protocol that allowed us to solve the 3.0 and 3.1 A resolution cryoelectron microscopy structures of these RNases poised to cleave their pre-rRNA substrates within the B. subtilis 50S particle. These data provide the first structural insights into rRNA maturation in bacteria by revealing how these RNases recognize and process double-stranded pre-rRNA. Our structures further uncover how specific ribosomal proteins act as chaperones to correctly fold the pre-rRNA substrates and, for Mini-III, anchor the RNase to the ribosome. These r-proteins thereby serve a quality-control function in the process from accurate ribosome assembly to rRNA processing. | ||
| + | |||
| + | Structures of B. subtilis Maturation RNases Captured on 50S Ribosome with Pre-rRNAs.,Oerum S, Dendooven T, Catala M, Gilet L, Degut C, Trinquier A, Bourguet M, Barraud P, Cianferani S, Luisi BF, Condon C, Tisne C Mol Cell. 2020 Sep 23. pii: S1097-2765(20)30616-X. doi:, 10.1016/j.molcel.2020.09.008. PMID:32991829<ref>PMID:32991829</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6tgj" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Atcc 12980]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Catala M]] | + | [[Category: Ribonuclease M5]] |
| - | [[Category: Degut C]] | + | [[Category: Catala, M]] |
| - | [[Category: Oerum S]] | + | [[Category: Degut, C]] |
| - | [[Category: Tisne C]] | + | [[Category: Oerum, S]] |
| + | [[Category: Tisne, C]] | ||
| + | [[Category: 5s rrna]] | ||
| + | [[Category: Novel fold]] | ||
| + | [[Category: Pre-5s rrna]] | ||
| + | [[Category: Precursor rrna]] | ||
| + | [[Category: Ribonuclease]] | ||
| + | [[Category: Ribosomal rna]] | ||
| + | [[Category: Rna binding protein]] | ||
| + | [[Category: Rna-binding domain]] | ||
| + | [[Category: Rnase]] | ||
| + | [[Category: Rnase m5]] | ||
| + | [[Category: Rrna]] | ||
Revision as of 06:14, 14 October 2020
RNA-binding domain of RNase M5
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Categories: Atcc 12980 | Large Structures | Ribonuclease M5 | Catala, M | Degut, C | Oerum, S | Tisne, C | 5s rrna | Novel fold | Pre-5s rrna | Precursor rrna | Ribonuclease | Ribosomal rna | Rna binding protein | Rna-binding domain | Rnase | Rnase m5 | Rrna
