7cri
From Proteopedia
(Difference between revisions)
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==1 ps Structure of Chloride ion pumping rhodopsin (ClR) with NTQ motif== | ==1 ps Structure of Chloride ion pumping rhodopsin (ClR) with NTQ motif== | ||
| - | <StructureSection load='7cri' size='340' side='right'caption='[[7cri]]' scene=''> | + | <StructureSection load='7cri' size='340' side='right'caption='[[7cri]], [[Resolution|resolution]] 1.85Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CRI OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[7cri]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Flavobacteria_bacterium_s1-08 Flavobacteria bacterium s1-08]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CRI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CRI FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ClR, NMS_1267 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1454201 Flavobacteria bacterium S1-08])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cri FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cri OCA], [https://pdbe.org/7cri PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cri RCSB], [https://www.ebi.ac.uk/pdbsum/7cri PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cri ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Chloride ion-pumping rhodopsin (ClR) in some marine bacteria utilizes light energy to actively transport Cl(-) into cells. How the ClR initiates the transport is elusive. Here, we show the dynamics of ion transport observed with time-resolved serial femtosecond (fs) crystallography using the Linac Coherent Light Source. X-ray pulses captured structural changes in ClR upon flash illumination with a 550 nm fs-pumping laser. High-resolution structures for five time points (dark to 100 ps after flashing) reveal complex and coordinated dynamics comprising retinal isomerization, water molecule rearrangement, and conformational changes of various residues. Combining data from time-resolved spectroscopy experiments and molecular dynamics simulations, this study reveals that the chloride ion close to the Schiff base undergoes a dissociation-diffusion process upon light-triggered retinal isomerization. | ||
| + | |||
| + | Early-stage dynamics of chloride ion-pumping rhodopsin revealed by a femtosecond X-ray laser.,Yun JH, Li X, Yue J, Park JH, Jin Z, Li C, Hu H, Shi Y, Pandey S, Carbajo S, Boutet S, Hunter MS, Liang M, Sierra RG, Lane TJ, Zhou L, Weierstall U, Zatsepin NA, Ohki M, Tame JRH, Park SY, Spence JCH, Zhang W, Schmidt M, Lee W, Liu H Proc Natl Acad Sci U S A. 2021 Mar 30;118(13). pii: 2020486118. doi:, 10.1073/pnas.2020486118. PMID:33753488<ref>PMID:33753488</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7cri" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Rhodopsin 3D structures|Rhodopsin 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Flavobacteria bacterium s1-08]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Lee | + | [[Category: Lee, W T]] |
| - | [[Category: Liu H]] | + | [[Category: Liu, H]] |
| - | [[Category: Schmidt M]] | + | [[Category: Schmidt, M]] |
| - | [[Category: Yun | + | [[Category: Yun, J H]] |
| + | [[Category: Chloride ion pumping rhodopsin]] | ||
| + | [[Category: Clr]] | ||
| + | [[Category: Membrane protein]] | ||
| + | [[Category: Ntq]] | ||
| + | [[Category: Sfx]] | ||
| + | [[Category: Xfel]] | ||
Revision as of 07:01, 14 April 2021
1 ps Structure of Chloride ion pumping rhodopsin (ClR) with NTQ motif
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