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| | <StructureSection load='5w1o' size='340' side='right'caption='[[5w1o]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='5w1o' size='340' side='right'caption='[[5w1o]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5w1o]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_papilloma_virus Human papilloma virus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3oae 3oae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W1O OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5W1O FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5w1o]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_papillomavirus Human papillomavirus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3oae 3oae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W1O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W1O FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IDS:2-O-SULFO-ALPHA-L-IDOPYRANURONIC+ACID'>IDS</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=JHM:2-DEOXY-6-O-SULFO-ALPHA-D-ARABINO-HEXOPYRANOSE'>JHM</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IDS:2-O-SULFO-ALPHA-L-IDOPYRANURONIC+ACID'>IDS</scene>, <scene name='pdbligand=JHM:2-DEOXY-6-O-SULFO-ALPHA-D-ARABINO-HEXOPYRANOSE'>JHM</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">L1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10566 Human Papilloma Virus])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w1o OCA], [https://pdbe.org/5w1o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w1o RCSB], [https://www.ebi.ac.uk/pdbsum/5w1o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w1o ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5w1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w1o OCA], [http://pdbe.org/5w1o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w1o RCSB], [http://www.ebi.ac.uk/pdbsum/5w1o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w1o ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q81007_9PAPI Q81007_9PAPI] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Major capsid protein L1|Major capsid protein L1]] | + | *[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human papilloma virus]] | |
| - | [[Category: Large Structures]] | |
| - | [[Category: Chen, X S]] | |
| - | [[Category: Dasgupta, J]] | |
| - | [[Category: Capsid protein]] | |
| | [[Category: Human papillomavirus]] | | [[Category: Human papillomavirus]] |
| - | [[Category: Jelly roll]] | + | [[Category: Large Structures]] |
| - | [[Category: Receptor hspg]] | + | [[Category: Chen XS]] |
| - | [[Category: Viral protein]] | + | [[Category: Dasgupta J]] |
| - | [[Category: Virus capsid]]
| + | |
| Structural highlights
Function
Q81007_9PAPI
Publication Abstract from PubMed
The high-risk human papillomavirus types 16 (HPV16) and 18 (HPV18) can cause cervical cancer. Efficient infection by HPV16 and 18 pseudovirions requires interactions ofparticles with cell surface receptor heparan sulfate oligosaccharide. To understand the virus-receptor interactions for HPV infection, we determined the crystal structures of HPV16 and 18 capsids bound to the oligosaccharide receptor fragment using oligomeric heparin. The HPV-heparin structures revealed multiple binding sites for the highly negatively charged oligosaccharide fragment on the capsid surface, which is different from previously reported virus-receptor interactions where a single type of binding pocket is present for a particular receptor. We performed structure-guided mutagenesis to generate mutant viruses, and the cell binding and infectivity assays demonstrated the functional role of viral residues involved in heparin binding. These results provide a basis for understanding virus-heparan sulfate receptor interactions critical for HPV infection, and for the potential development of inhibitors against HPV infection.
Structural basis of oligosaccharide receptor recognition by human Papillomavirus.,Dasgupta J, Bienkowska-Haba M, Ortega ME, Patel HD, Bodevin S, Spillmann D, Bishop B, Sapp M, Chen XS J Biol Chem. 2010 Nov 30. PMID:21115492[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Dasgupta J, Bienkowska-Haba M, Ortega ME, Patel HD, Bodevin S, Spillmann D, Bishop B, Sapp M, Chen XS. Structural basis of oligosaccharide receptor recognition by human Papillomavirus. J Biol Chem. 2010 Nov 30. PMID:21115492 doi:10.1074/jbc.M110.160184
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