5woz

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==NMR solution structure of Rtt103 (RTT) protein using two 4D-spectra==
==NMR solution structure of Rtt103 (RTT) protein using two 4D-spectra==
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<StructureSection load='5woz' size='340' side='right'caption='[[5woz]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
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<StructureSection load='5woz' size='340' side='right'caption='[[5woz]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5woz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WOZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WOZ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5woz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WOZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WOZ FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5woz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5woz OCA], [http://pdbe.org/5woz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5woz RCSB], [http://www.ebi.ac.uk/pdbsum/5woz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5woz ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5woz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5woz OCA], [https://pdbe.org/5woz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5woz RCSB], [https://www.ebi.ac.uk/pdbsum/5woz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5woz ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/RT103_YEAST RT103_YEAST]] Involved in transcription termination by RNA polymerase II and in regulation of Ty1 transposition.<ref>PMID:11779788</ref> <ref>PMID:15565157</ref>
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[https://www.uniprot.org/uniprot/RT103_YEAST RT103_YEAST] Involved in transcription termination by RNA polymerase II and in regulation of Ty1 transposition.<ref>PMID:11779788</ref> <ref>PMID:15565157</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Automated methods for NMR structure determination of proteins are continuously becoming more robust. However, current methods addressing larger, more complex targets rely on analyzing 6-10 complementary spectra, suggesting the need for alternative approaches. Here, we describe 4D-CHAINS/autoNOE-Rosetta, a complete pipeline for NOE-driven structure determination of medium- to larger-sized proteins. The 4D-CHAINS algorithm analyzes two 4D spectra recorded using a single, fully protonated protein sample in an iterative ansatz where common NOEs between different spin systems supplement conventional through-bond connectivities to establish assignments of sidechain and backbone resonances at high levels of completeness and with a minimum error rate. The 4D-CHAINS assignments are then used to guide automated assignment of long-range NOEs and structure refinement in autoNOE-Rosetta. Our results on four targets ranging in size from 15.5 to 27.3 kDa illustrate that the structures of proteins can be determined accurately and in an unsupervised manner in a matter of days.
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Automated NMR resonance assignments and structure determination using a minimal set of 4D spectra.,Evangelidis T, Nerli S, Novacek J, Brereton AE, Karplus PA, Dotas RR, Venditti V, Sgourakis NG, Tripsianes K Nat Commun. 2018 Jan 26;9(1):384. doi: 10.1038/s41467-017-02592-z. PMID:29374165<ref>PMID:29374165</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5woz" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Evangelidis, T]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Nerli, S]]
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[[Category: Evangelidis T]]
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[[Category: Sgourakis, N G]]
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[[Category: Nerli S]]
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[[Category: Tripsianes, K]]
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[[Category: Sgourakis NG]]
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[[Category: Rna polymerase ii core binder]]
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[[Category: Tripsianes K]]
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[[Category: Transcription regulator]]
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Current revision

NMR solution structure of Rtt103 (RTT) protein using two 4D-spectra

PDB ID 5woz

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