5wxb

From Proteopedia

(Difference between revisions)

Current revision

crystal structure of ZIKV MTase in complex with SAH

Structural highlights

5wxb is a 1 chain structure with sequence from Zika virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.762Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A167V0D5_ZIKV

Publication Abstract from PubMed

An outbreak of Zika virus (ZIKV) infection has been reported in South and Central America and the Caribbean. Neonatal microcephaly potentially associated with ZIKV infection has already caused a public health emergency of international concern. Currently, there are no clinically effective vaccines or antiviral drugs available to treat ZIKV infection. The methyltransferase domain (MTase) of ZIKV nonstructural protein 5 (NS5) can sequentially methylate guanine N-7 and ribose 2'-O to form m7NGpppA2'Om cap structure in the new RNA transcripts. This methylation step is crucial for ZIKV replication cycle and evading the host immune system, making it a target for drug design. Here, we present the 1.76 A crystal structure of ZIKV MTase in complex with the byproduct SAH, providing insight into the elegant methylation process, which will benefit the following antiviral drug development.

The conformational changes of Zika virus methyltransferase upon converting SAM to SAH.,Zhou H, Wang F, Wang H, Chen C, Zhang T, Han X, Wang D, Chen C, Wu C, Xie W, Wang Z, Zhang L, Wang L, Yang H Oncotarget. 2017 Feb 28;8(9):14830-14834. doi: 10.18632/oncotarget.14780. PMID:28122329^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhou H, Wang F, Wang H, Chen C, Zhang T, Han X, Wang D, Chen C, Wu C, Xie W, Wang Z, Zhang L, Wang L, Yang H. The conformational changes of Zika virus methyltransferase upon converting SAM to SAH. Oncotarget. 2017 Feb 28;8(9):14830-14834. doi: 10.18632/oncotarget.14780. PMID:28122329 doi:http://dx.doi.org/10.18632/oncotarget.14780

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5wxb"

Categories: Large Structures | Zika virus | Wang L | Yang H | Zhou H

@@ Line 3: / Line 3: @@
 <StructureSection load='5wxb' size='340' side='right'caption='[[5wxb]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5wxb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zikv Zikv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WXB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WXB FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5wxb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zika_virus Zika virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WXB FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.762&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GP1, A2G93_63486gpGP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=64320 ZIKV])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wxb OCA], [http://pdbe.org/5wxb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wxb RCSB], [http://www.ebi.ac.uk/pdbsum/5wxb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wxb ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wxb OCA], [https://pdbe.org/5wxb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wxb RCSB], [https://www.ebi.ac.uk/pdbsum/5wxb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wxb ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A167V0D5_ZIKV A0A167V0D5_ZIKV]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 24: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Zikv]]
-[[Category: Wang, L]]
-[[Category: Yang, H]]
-[[Category: Zhou, H]]
-[[Category: Methyltransferase]]
-[[Category: Sah]]
-[[Category: Transferase]]
 [[Category: Zika virus]]
+[[Category: Wang L]]
+[[Category: Yang H]]
+[[Category: Zhou H]]

5wxb

From Proteopedia

Current revision

crystal structure of ZIKV MTase in complex with SAH

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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