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1cs1

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{{STRUCTURE_1cs1| PDB=1cs1 | SCENE= }}
{{STRUCTURE_1cs1| PDB=1cs1 | SCENE= }}
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'''CYSTATHIONINE GAMMA-SYNTHASE (CGS) FROM ESCHERICHIA COLI'''
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===CYSTATHIONINE GAMMA-SYNTHASE (CGS) FROM ESCHERICHIA COLI===
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==Overview==
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The transsulfuration enzyme cystathionine gamma-synthase (CGS) catalyses the pyridoxal 5'-phosphate (PLP)-dependent gamma-replacement of O-succinyl-L-homoserine and L-cysteine, yielding L-cystathionine. The crystal structure of the Escherichia coli enzyme has been solved by molecular replacement with the known structure of cystathionine beta-lyase (CBL), and refined at 1.5 A resolution to a crystallographic R-factor of 20.0%. The enzyme crystallizes as an alpha4 tetramer with the subunits related by non-crystallographic 222 symmetry. The spatial fold of the subunits, with three functionally distinct domains and their quaternary arrangement, is similar to that of CBL. Previously proposed reaction mechanisms for CGS can be checked against the structural model, allowing interpretation of the catalytic and substrate-binding functions of individual active site residues. Enzyme-substrate models pinpoint specific residues responsible for the substrate specificity, in agreement with structural comparisons with CBL. Both steric and electrostatic designs of the active site seem to achieve proper substrate selection and productive orientation. Amino acid sequence and structural alignments of CGS and CBL suggest that differences in the substrate-binding characteristics are responsible for the different reaction chemistries. Because CGS catalyses the only known PLP-dependent replacement reaction at Cgamma of certain amino acids, the results will help in our understanding of the chemical versatility of PLP.
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(as it appears on PubMed at http://www.pubmed.gov), where 9843488 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9843488}}
==About this Structure==
==About this Structure==
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[[Category: Lyase]]
[[Category: Lyase]]
[[Category: Methionine biosynthesis]]
[[Category: Methionine biosynthesis]]
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Revision as of 18:15, 30 June 2008

Image:1cs1.png

Template:STRUCTURE 1cs1

CYSTATHIONINE GAMMA-SYNTHASE (CGS) FROM ESCHERICHIA COLI

Template:ABSTRACT PUBMED 9843488

About this Structure

1CS1 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of Escherichia coli cystathionine gamma-synthase at 1.5 A resolution., Clausen T, Huber R, Prade L, Wahl MC, Messerschmidt A, EMBO J. 1998 Dec 1;17(23):6827-38. PMID:9843488

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