6nwy
From Proteopedia
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| - | ==== | + | ==Modified tRNA(Pro) bound to Thermus thermophilus 70S (near-cognate)== |
| - | <StructureSection load='6nwy' size='340' side='right'caption='[[6nwy]]' scene=''> | + | <StructureSection load='6nwy' size='340' side='right'caption='[[6nwy]], [[Resolution|resolution]] 3.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6nwy]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NWY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NWY FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1MG:1N-METHYLGUANOSINE-5-MONOPHOSPHATE'>1MG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nwy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nwy OCA], [https://pdbe.org/6nwy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nwy RCSB], [https://www.ebi.ac.uk/pdbsum/6nwy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nwy ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RS4_THET8 RS4_THET8] One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the body and platform of the 30S subunit. Binds mRNA in the 70S ribosome, positioning it for translation.[HAMAP-Rule:MF_01306_B] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Modifications in the tRNA anticodon loop, adjacent to the three-nucleotide anticodon, influence translation fidelity by stabilizing the tRNA to allow for accurate reading of the mRNA genetic code. One example is the N1-methylguaonosine modification at guanine nucleotide 37 (m(1)G37) located in the anticodon loop, immediately adjacent to the anticodon nucleotides 34-36. The absence of m(1)G37 in tRNA(Pro) causes +1 frameshifting on polynucleotide, slippery codons. Here, we report structures of the bacterial ribosome containing tRNA(Pro) bound to either cognate or slippery codons to determine how the m(1)G37 modification prevents mRNA frameshifting. The structures reveal that certain codon-anticodon contexts and m(1)G37 destabilize interactions of tRNA(Pro) with the peptidyl site, causing large conformational changes typically only seen during EF-G mediated translocation of the mRNA-tRNA pairs. These studies provide molecular insights into how m(1)G37 stabilizes the interactions of tRNA(Pro) with the ribosome and the influence of slippery codons on the mRNA reading frame. | ||
| + | |||
| + | Structural insights into mRNA reading frame regulation by tRNA modification and slippery codon-anticodon pairing.,Hoffer E, Hong S, Sunita S, Maehigashi T, Gonzalez RL Jnr, Whitford P, Dunham CM Elife. 2020 Oct 5;9. pii: 51898. doi: 10.7554/eLife.51898. PMID:33016876<ref>PMID:33016876</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6nwy" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Escherichia coli]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Thermus thermophilus HB8]] |
| + | [[Category: Dunham CM]] | ||
| + | [[Category: Hoffer ED]] | ||
| + | [[Category: Hong S]] | ||
| + | [[Category: Maehigashi T]] | ||
| + | [[Category: Subaramanian S]] | ||
Revision as of 07:01, 11 October 2023
Modified tRNA(Pro) bound to Thermus thermophilus 70S (near-cognate)
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