This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
6zyk
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Non-heme monooxygenase, ThoJ-Ni complex== | ==Non-heme monooxygenase, ThoJ-Ni complex== | ||
| - | <StructureSection load='6zyk' size='340' side='right'caption='[[6zyk]]' scene=''> | + | <StructureSection load='6zyk' size='340' side='right'caption='[[6zyk]], [[Resolution|resolution]] 2.55Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZYK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZYK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6zyk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptomyces_malaysiense"_ser_et_al._2016 "streptomyces malaysiense" ser et al. 2016]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZYK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZYK FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zyk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zyk OCA], [http://pdbe.org/6zyk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zyk RCSB], [http://www.ebi.ac.uk/pdbsum/6zyk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zyk ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VT52_024865 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1428626 "Streptomyces malaysiense" Ser et al. 2016])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zyk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zyk OCA], [http://pdbe.org/6zyk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zyk RCSB], [http://www.ebi.ac.uk/pdbsum/6zyk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zyk ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Thioviridamide-like compounds, including thioholgamides, are ribosomally synthesized and post-translationally modified peptide natural products with potent anticancer cell activity and an unprecedented structure. Very little is known about their biosynthesis, and we were intrigued by the beta-hydroxy-N1, N3-dimethylhistidinium moiety found in these compounds. Here we report the construction of a heterologous host capable of producing thioholgamide with a 15-fold increased yield compared to the wild-type strain. A knockout of thoJ, encoding a predicted nonheme monooxygenase, shows that ThoJ is essential for thioholgamide beta-hydroxylation. The crystal structure of ThoJ exhibits a typical mono/dioxygenase fold with conserved key active-site residues. Yet, ThoJ possesses a very large substrate binding pocket that appears suitable to receive a cyclic thioholgamide intermediate for hydroxylation. The improved production of the heterologous host will enable the dissection of the individual biosynthetic steps involved in biosynthesis of this exciting RiPP family. | ||
| + | |||
| + | Non-Heme Monooxygenase ThoJ Catalyzes Thioholgamide beta-Hydroxylation.,Sikandar A, Lopatniuk M, Luzhetskyy A, Koehnke J ACS Chem Biol. 2020 Oct 1. doi: 10.1021/acschembio.0c00637. PMID:32965102<ref>PMID:32965102</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6zyk" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Streptomyces malaysiense ser et al. 2016]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Koehnke J]] | + | [[Category: Koehnke, J]] |
| - | [[Category: Sikandar A]] | + | [[Category: Sikandar, A]] |
| + | [[Category: Metal binding protein]] | ||
| + | [[Category: Non-heme monooxygenase]] | ||
Revision as of 20:54, 28 October 2020
Non-heme monooxygenase, ThoJ-Ni complex
| |||||||||||
