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5xja

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The Crystal Structure of the Minimal Core Domain of the Microtubule Depolymerizer KIF2C Complexed with ADP-Mg-AlFx

Structural highlights

5xja is a 2 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.43Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

KIF2C_MOUSE In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells (By similarity). Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis (By similarity).

Publication Abstract from PubMed

Microtubules (MTs) are dynamic structures that are fundamental for cell morphogenesis and motility. MT-associated motors work efficiently to perform their functions. Unlike other motile kinesins, KIF2 catalytically depolymerizes MTs from the peeled protofilament end during ATP hydrolysis. However, the detailed mechanism by which KIF2 drives processive MT depolymerization remains unknown. To elucidate the catalytic mechanism, the transitional KIF2-tubulin complex during MT depolymerization was analyzed through multiple methods, including atomic force microscopy, size-exclusion chromatography, multi-angle light scattering, small-angle X-ray scattering, analytical ultracentrifugation, and mass spectrometry. The analyses outlined the conformation in which one KIF2core domain binds tightly to two tubulin dimers in the middle pre-hydrolysis state during ATP hydrolysis, a process critical for catalytic MT depolymerization. The X-ray crystallographic structure of the KIF2core domain displays the activated conformation that sustains the large KIF2-tubulin 1:2 complex.

Mechanism of Catalytic Microtubule Depolymerization via KIF2-Tubulin Transitional Conformation.,Ogawa T, Saijo S, Shimizu N, Jiang X, Hirokawa N Cell Rep. 2017 Sep 12;20(11):2626-2638. doi: 10.1016/j.celrep.2017.08.067. PMID:28903043^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ogawa T, Saijo S, Shimizu N, Jiang X, Hirokawa N. Mechanism of Catalytic Microtubule Depolymerization via KIF2-Tubulin Transitional Conformation. Cell Rep. 2017 Sep 12;20(11):2626-2638. doi: 10.1016/j.celrep.2017.08.067. PMID:28903043 doi:http://dx.doi.org/10.1016/j.celrep.2017.08.067

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5xja"

Categories: Large Structures | Mus musculus | Hirokawa N | Jiang X | Ogawa T

@@ Line 3: / Line 3: @@
 <StructureSection load='5xja' size='340' side='right'caption='[[5xja]], [[Resolution|resolution]] 3.43&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5xja]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XJA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5XJA FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5xja]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XJA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XJA FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.43&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5xjb|5xjb]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5xja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xja OCA], [http://pdbe.org/5xja PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xja RCSB], [http://www.ebi.ac.uk/pdbsum/5xja PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xja ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xja OCA], [https://pdbe.org/5xja PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xja RCSB], [https://www.ebi.ac.uk/pdbsum/5xja PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xja ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/KIF2C_MOUSE KIF2C_MOUSE]] In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells (By similarity). Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis (By similarity).
+[https://www.uniprot.org/uniprot/KIF2C_MOUSE KIF2C_MOUSE] In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells (By similarity). Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis (By similarity).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 27: / Line 27: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Hirokawa, N]]
+[[Category: Mus musculus]]
-[[Category: Jiang, X]]
+[[Category: Hirokawa N]]
-[[Category: Ogawa, T]]
+[[Category: Jiang X]]
-[[Category: Depolymerization]]
+[[Category: Ogawa T]]
-[[Category: Kif2]]
-[[Category: Kinesin]]
-[[Category: Mcak]]
-[[Category: Microtubule]]
-[[Category: Structural protein]]
-[[Category: Tubulin]]

5xja

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Current revision

The Crystal Structure of the Minimal Core Domain of the Microtubule Depolymerizer KIF2C Complexed with ADP-Mg-AlFx

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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