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| | <StructureSection load='5y6j' size='340' side='right'caption='[[5y6j]], [[Resolution|resolution]] 2.81Å' scene=''> | | <StructureSection load='5y6j' size='340' side='right'caption='[[5y6j]], [[Resolution|resolution]] 2.81Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5y6j]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y6J OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5Y6J FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5y6j]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Tomato_spotted_wilt_orthotospovirus Tomato spotted wilt orthotospovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y6J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Y6J FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5y6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y6j OCA], [http://pdbe.org/5y6j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y6j RCSB], [http://www.ebi.ac.uk/pdbsum/5y6j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y6j ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.811Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5y6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y6j OCA], [https://pdbe.org/5y6j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5y6j RCSB], [https://www.ebi.ac.uk/pdbsum/5y6j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5y6j ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/H6UMW9_TSWV H6UMW9_TSWV] |
| - | The tomato spotted wilt virus (TSWV) belongs to the Tospovirus genus of the Bunyaviridae family and represents the sole plant-infecting group within bunyavirus. TSWV encodes a nucleocapsid protein (N) which encapsidates the RNA genome to form a ribonucleoprotein complex (RNP). In addition, the N has multiple roles during the infection of plant cells. Here, we report the crystal structure of the full-length TSWV N. The N features a body domain consisting of an N- and C-lobe. These lobes clamp a positively charged groove which may constitute the RNA binding site. Furthermore, the body domains are flanked by N- and C-terminal arms which mediate homotypic interactions to the neighboring subunits, resulting in a ring-shaped N trimer. Interestingly, the C-terminus of one protomer forms an additional interaction with the protomer of an adjacent trimer in the crystal, which may constitute a higher-order oligomerization contact. In this way, this study provides insights into the structure and trimeric assembly of TSWV N which help to explain previous functional findings, but also suggests distinct N interactions within a higher-order RNP.Importance TSWV is one of the most devastating plant pathogens that cause severe diseases in numerous agronomic and ornamental crops worldwide. TSWV is also the prototypic member of the Tospovirus genus, which is the sole group of plant-infecting viruses in bunyavirus family. This study determined the structure of full-length TSWV N in an oligomeric state. The structural observations explain previously identified biological properties of TSWV N. Most importantly, the additional homotypic interaction between the C-terminus of one protomer with another protomer indicates there is a distinct mechanism of RNP formation in the bunyavirus family, thereby enhancing current knowledge of --ssRNA virus-encoded N. TSWV N is the last remaining representative N with an unknown structure in the bunyavirus family. Combined with previous studies, the structure of TSWV N helps to build a complete picture of the bunyavirus-encoded N family and reveals a close evolutionary relationship between orthobunyavirus, phlebovirus, hantavirus and tospovirus.
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| - | A distinct mechanism for the formation of the ribonucleoprotein complex of the Tomato spotted wilt virus.,Guo Y, Liu B, Ding Z, Li G, Liu M, Zhu D, Sun Y, Dong S, Lou Z J Virol. 2017 Sep 13. pii: JVI.00892-17. doi: 10.1128/JVI.00892-17. PMID:28904194<ref>PMID:28904194</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5y6j" style="background-color:#fffaf0;"></div>
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| | | | |
| | ==See Also== | | ==See Also== |
| | *[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] | | *[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] |
| - | == References == | |
| - | <references/> | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Dong, S]] | + | [[Category: Tomato spotted wilt orthotospovirus]] |
| - | [[Category: Guo, Y]] | + | [[Category: Dong S]] |
| - | [[Category: Lou, Z]] | + | [[Category: Guo Y]] |
| - | [[Category: Np]] | + | [[Category: Lou Z]] |
| - | [[Category: Nucleocapsid protein]]
| + | |
| - | [[Category: Oligomerization]]
| + | |
| - | [[Category: Tswv]]
| + | |
| - | [[Category: Viral protein]]
| + | |
