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| | <StructureSection load='6oiu' size='340' side='right'caption='[[6oiu]], [[Resolution|resolution]] 2.20Å' scene=''> | | <StructureSection load='6oiu' size='340' side='right'caption='[[6oiu]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6oiu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxgm Toxgm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OIU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OIU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6oiu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxoplasma_gondii_ME49 Toxoplasma gondii ME49]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OIU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OIU FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TGME49_224350 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=508771 TOXGM])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Membrane_alanyl_aminopeptidase Membrane alanyl aminopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.11.2 3.4.11.2] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6oiu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oiu OCA], [https://pdbe.org/6oiu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6oiu RCSB], [https://www.ebi.ac.uk/pdbsum/6oiu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6oiu ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6oiu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oiu OCA], [http://pdbe.org/6oiu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oiu RCSB], [http://www.ebi.ac.uk/pdbsum/6oiu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oiu ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/S8G5K8_TOXGM S8G5K8_TOXGM] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6oiu" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6oiu" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Aminopeptidase 3D structures|Aminopeptidase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Membrane alanyl aminopeptidase]] | + | [[Category: Toxoplasma gondii ME49]] |
| - | [[Category: Toxgm]]
| + | [[Category: Drinkwater N]] |
| - | [[Category: Drinkwater, N]] | + | [[Category: McGowan S]] |
| - | [[Category: McGowan, S]] | + | |
| - | [[Category: Aminopeptidase n]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: M1 aminopeptidase]]
| + | |
| - | [[Category: Metallo-exopeptidase]]
| + | |
| Structural highlights
Function
S8G5K8_TOXGM
Publication Abstract from PubMed
Toxoplasmosis is a parasitic disease caused by infection with Toxoplasma gondii that currently has few therapeutic options. The M1 aminopeptidase enzymes have been shown to be attractive targets for anti-parasitic agents and / or vaccine candidates, suggesting potential to re-purpose inhibitors between parasite M1 aminopeptidase targets. The M1 aminopeptidase TgAPN2 has been suggested to be a potential new drug target for toxoplasmosis. Here we investigate the structure and function of TgAPN2, a homolog of the antimalarial drug target PfA-M1, and evaluate the capacity to use inhibitors that target PfA-M1 against TgAPN2. The results show that despite a similar overall fold, the TgAPN2 has a unique substrate specificity and inhibition profile. Sequence and structure differences are investigated and show how comparative structure-activity relationships may provide a route to obtaining potent inhibitors of TgAPN2.
X-ray crystal structure and specificity of the Toxoplasma gondii ME49 TgAPN2.,Marijanovic EM, Weronika Swiderka K, Andersen J, Aschenbrenner JC, Webb CT, Drag M, Drinkwater N, McGowan S Biochem J. 2020 Sep 14. pii: 226405. doi: 10.1042/BCJ20200569. PMID:32926129[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Marijanovic EM, Weronika Swiderka K, Andersen J, Aschenbrenner JC, Webb CT, Drag M, Drinkwater N, McGowan S. X-ray crystal structure and specificity of the Toxoplasma gondii ME49 TgAPN2. Biochem J. 2020 Sep 14. pii: 226405. doi: 10.1042/BCJ20200569. PMID:32926129 doi:http://dx.doi.org/10.1042/BCJ20200569
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