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6z7u

From Proteopedia

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Current revision

Myosin-II motor domain complexed with blebbistatin in a new ADP-release conformation

Structural highlights

6z7u is a 1 chain structure with sequence from Dictyostelium discoideum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.58Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MYS2_DICDI Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.

Publication Abstract from PubMed

The motor protein myosin drives a wide range of cellular and muscular functions by generating directed movement and force, fueled through adenosine triphosphate (ATP) hydrolysis. Release of the hydrolysis product adenosine diphosphate (ADP) is a fundamental and regulatory process during force production. However, details about the molecular mechanism accompanying ADP release are scarce due to the lack of representative structures. Here we solved a novel blebbistatin-bound myosin conformation with critical structural elements in positions between the myosin pre-power stroke and rigor states. ADP in this structure is repositioned towards the surface by the phosphate-sensing P-loop, and stabilized in a partially unbound conformation via a salt-bridge between Arg131 and Glu187. A 5 A rotation separates the mechanical converter in this conformation from the rigor position. The crystallized myosin structure thus resembles a conformation towards the end of the two-step power stroke, associated with ADP release. Computationally reconstructing ADP release from myosin by means of molecular dynamics simulations further supported the existence of an equivalent conformation along the power stroke that shows the same major characteristics in the myosin motor domain as the resolved blebbistatin-bound myosin-II.ADP crystal structure, and identified a communication hub centered on Arg232 that mediates chemomechanical energy transduction.

Structural and Computational Insights into a Blebbistatin-Bound Myosin*ADP Complex with Characteristics of an ADP-Release Conformation along the Two-Step Myosin Power Stoke.,Ewert W, Franz P, Tsiavaliaris G, Preller M Int J Mol Sci. 2020 Oct 8;21(19). pii: ijms21197417. doi: 10.3390/ijms21197417. PMID:33049993^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ewert W, Franz P, Tsiavaliaris G, Preller M. Structural and Computational Insights into a Blebbistatin-Bound Myosin*ADP Complex with Characteristics of an ADP-Release Conformation along the Two-Step Myosin Power Stoke. Int J Mol Sci. 2020 Oct 8;21(19). pii: ijms21197417. doi: 10.3390/ijms21197417. PMID:33049993 doi:http://dx.doi.org/10.3390/ijms21197417

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6z7u"

Categories: Dictyostelium discoideum | Large Structures | Ewert W | Preller M

@@ Line 3: / Line 3: @@
 <StructureSection load='6z7u' size='340' side='right'caption='[[6z7u]], [[Resolution|resolution]] 2.58&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6z7u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dicdi Dicdi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z7U OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Z7U FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6z7u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z7U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z7U FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BIT:(-)-1-PHENYL-1,2,3,4-TETRAHYDRO-4-HYDROXYPYRROLO[2,3-B]-7-METHYLQUINOLIN-4-ONE'>BIT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.58&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mhcA, DDB_G0286355 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 DICDI])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BIT:(-)-1-PHENYL-1,2,3,4-TETRAHYDRO-4-HYDROXYPYRROLO[2,3-B]-7-METHYLQUINOLIN-4-ONE'>BIT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6z7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z7u OCA], [http://pdbe.org/6z7u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6z7u RCSB], [http://www.ebi.ac.uk/pdbsum/6z7u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6z7u ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z7u OCA], [https://pdbe.org/6z7u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z7u RCSB], [https://www.ebi.ac.uk/pdbsum/6z7u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z7u ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI]] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.
+[https://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 19: @@
 </div>
 <div class="pdbe-citations 6z7u" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Myosin 3D Structures|Myosin 3D Structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Dicdi]]
+[[Category: Dictyostelium discoideum]]
 [[Category: Large Structures]]
-[[Category: Ewert, W]]
+[[Category: Ewert W]]
-[[Category: Preller, M]]
+[[Category: Preller M]]
-[[Category: Adp-release]]
-[[Category: Blebbistatin]]
-[[Category: Complex]]
-[[Category: Hydrolase]]
-[[Category: Inhibitor]]
-[[Category: Motor protein]]
-[[Category: Motorprotein]]
-[[Category: Myosin]]

6z7u

From Proteopedia

Current revision

Myosin-II motor domain complexed with blebbistatin in a new ADP-release conformation

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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