|
|
| Line 3: |
Line 3: |
| | <StructureSection load='6zjm' size='340' side='right'caption='[[6zjm]], [[Resolution|resolution]] 11.40Å' scene=''> | | <StructureSection load='6zjm' size='340' side='right'caption='[[6zjm]], [[Resolution|resolution]] 11.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6zjm]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Tula_hantavirus Tula hantavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZJM OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZJM FirstGlance]. <br> | + | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZJM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZJM FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 11.4Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zjm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zjm OCA], [http://pdbe.org/6zjm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zjm RCSB], [http://www.ebi.ac.uk/pdbsum/6zjm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zjm ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zjm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zjm OCA], [https://pdbe.org/6zjm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zjm RCSB], [https://www.ebi.ac.uk/pdbsum/6zjm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zjm ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| Line 21: |
Line 22: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Tula hantavirus]]
| + | [[Category: Guardado-Calvo P]] |
| - | [[Category: Guardado-Calvo, P]] | + | [[Category: Huiskonen JT]] |
| - | [[Category: Huiskonen, J T]] | + | [[Category: Rey F]] |
| - | [[Category: Rey, F]] | + | [[Category: Stass R]] |
| - | [[Category: Stass, R]] | + | |
| - | [[Category: Ande]]
| + | |
| - | [[Category: Andv]]
| + | |
| - | [[Category: Fusion protein]]
| + | |
| - | [[Category: Glycoprotein]]
| + | |
| - | [[Category: Hantavirus]]
| + | |
| - | [[Category: Lattice]]
| + | |
| - | [[Category: Spike]]
| + | |
| - | [[Category: Tula]]
| + | |
| - | [[Category: Tulv]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Hantaviruses are rodent-borne viruses causing serious zoonotic outbreaks worldwide for which no treatment is available. Hantavirus particles are pleomorphic and display a characteristic square surface lattice. The envelope glycoproteins Gn and Gc form heterodimers that further assemble into tetrameric spikes, the lattice building blocks. The glycoproteins, which are the sole targets of neutralizing antibodies, drive virus entry via receptor-mediated endocytosis and endosomal membrane fusion. Here we describe the high-resolution X-ray structures of the heterodimer of Gc and the Gn head and of the homotetrameric Gn base. Docking them into an 11.4-A-resolution cryoelectron tomography map of the hantavirus surface accounted for the complete extramembrane portion of the viral glycoprotein shell and allowed a detailed description of the surface organization of these pleomorphic virions. Our results, which further revealed a built-in mechanism controlling Gc membrane insertion for fusion, pave the way for immunogen design to protect against pathogenic hantaviruses.
The Hantavirus Surface Glycoprotein Lattice and Its Fusion Control Mechanism.,Serris A, Stass R, Bignon EA, Muena NA, Manuguerra JC, Jangra RK, Li S, Chandran K, Tischler ND, Huiskonen JT, Rey FA, Guardado-Calvo P Cell. 2020 Sep 9. pii: S0092-8674(20)31064-3. doi: 10.1016/j.cell.2020.08.023. PMID:32937107[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Serris A, Stass R, Bignon EA, Muena NA, Manuguerra JC, Jangra RK, Li S, Chandran K, Tischler ND, Huiskonen JT, Rey FA, Guardado-Calvo P. The Hantavirus Surface Glycoprotein Lattice and Its Fusion Control Mechanism. Cell. 2020 Sep 9. pii: S0092-8674(20)31064-3. doi: 10.1016/j.cell.2020.08.023. PMID:32937107 doi:http://dx.doi.org/10.1016/j.cell.2020.08.023
|
