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6kx9

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Crystal structure of 8-mer peptide from avian influenza H5N1 virus in complex with BF2*1501

Structural highlights

6kx9 is a 3 chain structure with sequence from Gallus gallus and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.902Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9GIP6_CHICK

Publication Abstract from PubMed

Lethal infections by strains of the highly-pathogenic avian influenza virus (HPAIV) H5N1 pose serious threats to both the poultry industry and public health worldwide. A lack of confirmed HPAIV epitopes recognized by cytotoxic T lymphocytes (CTLs) has hindered the utilization of CD8(+) T-cell-mediated immunity and has precluded the development of effectively diversified epitope-based vaccination approaches. In particular, an HPAIV H5N1 CTL-recognized epitope based on the peptide MHC-I-beta2m (pMHC-I) complex has not yet been designed. Here, screening a collection of selected peptides of several HPAIV strains against a specific pathogen-free pMHC-I (pBF2*1501), we identified a highly-conserved HPAIV H5N1 CTL epitope, named HPAIV-PA123-130 We determined the structure of the BF2*1501-PA123-130 complex at 2.1 A resolution to elucidate the molecular mechanisms of a preferential presentation of the highly-conserved PA123-130 epitope in the chicken B15 lineage. Conformational characteristics of the PA123-130 epitope with a protruding Tyr-7 residue indicated that this epitope has great potential to be recognized by specific TCRs. Moreover, significantly increased numbers of CD8(+) T cells specific for the HPAIV-PA123-130 epitope in peptide-immunized chickens indicated that a repertoire of CD8(+) T cells can specifically respond to this epitope. We anticipate that the identification and structural characterization of the PA123-130 epitope reported here could enable further studies of CTL immunity against HPAIV H5N1. Such studies may aid in the development of vaccine development strategies using well-conserved internal viral antigens in chickens.

Structures of the MHC-I molecule BF2*1501 disclose the preferred presentation of an H5N1 virus-derived epitope.,Li X, Zhang L, Liu Y, Ma L, Zhang N, Xia C J Biol Chem. 2020 Apr 17;295(16):5292-5306. doi: 10.1074/jbc.RA120.012713. Epub, 2020 Mar 9. PMID:32152225^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Li X, Zhang L, Liu Y, Ma L, Zhang N, Xia C. Structures of the MHC-I molecule BF2*1501 disclose the preferred presentation of an H5N1 virus-derived epitope. J Biol Chem. 2020 Apr 17;295(16):5292-5306. doi: 10.1074/jbc.RA120.012713. Epub, 2020 Mar 9. PMID:32152225 doi:http://dx.doi.org/10.1074/jbc.RA120.012713

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6kx9"

Categories: Gallus gallus | Large Structures | Synthetic construct | Xiao L | Zhang L

@@ Line 3: / Line 3: @@
 <StructureSection load='6kx9' size='340' side='right'caption='[[6kx9]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6kx9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KX9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KX9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6kx9]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KX9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KX9 FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BF2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK]), B2M ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.902&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kx9 OCA], [http://pdbe.org/6kx9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kx9 RCSB], [http://www.ebi.ac.uk/pdbsum/6kx9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kx9 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kx9 OCA], [https://pdbe.org/6kx9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kx9 RCSB], [https://www.ebi.ac.uk/pdbsum/6kx9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kx9 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/B2MG_CHICK B2MG_CHICK]] Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
+[https://www.uniprot.org/uniprot/Q9GIP6_CHICK Q9GIP6_CHICK]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 21: @@
 ==See Also==
 *[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]]
+*[[MHC 3D structures|MHC 3D structures]]
+*[[MHC I 3D structures|MHC I 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Chick]]
+[[Category: Gallus gallus]]
 [[Category: Large Structures]]
-[[Category: Xiao, L]]
+[[Category: Synthetic construct]]
-[[Category: Zhang, L]]
+[[Category: Xiao L]]
-[[Category: 8-mer]]
+[[Category: Zhang L]]
-[[Category: Avian influenza virus]]
-[[Category: Chicken]]
-[[Category: Epitope]]
-[[Category: H5n1]]
-[[Category: Immune system]]
-[[Category: Mhc class i]]

6kx9

From Proteopedia

Current revision

Crystal structure of 8-mer peptide from avian influenza H5N1 virus in complex with BF2*1501

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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