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1czu

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{{STRUCTURE_1czu| PDB=1czu | SCENE= }}
{{STRUCTURE_1czu| PDB=1czu | SCENE= }}
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'''REFINED STRUCTURES OF OXIDIZED FLAVODOXIN FROM ANACYSTIS NIDULANS'''
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===REFINED STRUCTURES OF OXIDIZED FLAVODOXIN FROM ANACYSTIS NIDULANS===
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==Overview==
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Flavodoxin from Anacystis nidulans (Synechococcus PCC 7942) was the first member of the flavodoxin family to be characterized, and is the structural prototype for the "long-chain" flavodoxins that have molecular masses of approximately 20 kDa. Crystal structure analyses and refinements of three orthorhombic forms of oxidized A. nidulans flavodoxin are reported, and salient features of the fold and the FMN binding site are compared with other flavodoxins. The structure of form I (wild-type: P212121, a=57.08 A, b=69.24 A, c=45.55 A), determined initially by multiple isomorphous replacement, has been refined to R=0.183 and R(free)=0.211 for data from 10.0 to 1.7 A resolution. Structures of form II (wild-type: P212121, a=60.05 A, b=65.85 A, c=51.36 A) and form III (Asn58Gly: P212121, a=51.30 A, b=59.15 A, c=94.44 A) have been determined by molecular replacement and refined versus data to 2.0 A and 1.85 A, respectively; the R values for forms II and III are 0.147 and 0.150. Changes in the molecular contacts that produce the alternative packings in these crystalline forms are analyzed. Deletion of the Asn side-chain in the mutant Asn58Gly removes an intermolecular stacking interaction and allows the alternative packing found in form III crystals. The functionally important 50's loop of the FMN binding site is less restrained by intermolecular contacts in these crystals but maintains the same conformation as in oxidized wild type protein. The structures reported here provide the starting point for structure-function studies of the reduced states and of mutants, described in the accompanying paper.
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(as it appears on PubMed at http://www.pubmed.gov), where 10610791 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10610791}}
==About this Structure==
==About this Structure==
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[[Category: Fmn binding]]
[[Category: Fmn binding]]
[[Category: Redox potential]]
[[Category: Redox potential]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:17:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 21:53:30 2008''

Revision as of 18:53, 30 June 2008

Image:1czu.png

Template:STRUCTURE 1czu

REFINED STRUCTURES OF OXIDIZED FLAVODOXIN FROM ANACYSTIS NIDULANS

Template:ABSTRACT PUBMED 10610791

About this Structure

1CZU is a Single protein structure of sequence from Synechococcus sp.. Full crystallographic information is available from OCA.

Reference

Refined structures of oxidized flavodoxin from Anacystis nidulans., Drennan CL, Pattridge KA, Weber CH, Metzger AL, Hoover DM, Ludwig ML, J Mol Biol. 1999 Dec 3;294(3):711-24. PMID:10610791

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