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1d0t

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[[Image:1d0t.gif|left|200px]]
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{{Seed}}
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[[Image:1d0t.png|left|200px]]
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{{STRUCTURE_1d0t| PDB=1d0t | SCENE= }}
{{STRUCTURE_1d0t| PDB=1d0t | SCENE= }}
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'''SOLUTION STRUCTURE OF A PHOSPHOROTHIOATE MODIFIED RNA BINDING SITE FOR PHAGE MS2 COAT PROTEIN'''
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===SOLUTION STRUCTURE OF A PHOSPHOROTHIOATE MODIFIED RNA BINDING SITE FOR PHAGE MS2 COAT PROTEIN===
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==Overview==
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Phosphorothioate substitution-interference experiments, routinely used to stereospecifically identify phosphoryl oxygen sites that participate in RNA-ligand binding and RNA-directed catalysis, rest in their interpretation on the untested assumption that substitution does not alter the conformation of the modified molecule from its biologically active state. Using NMR spectroscopy, we have tested this assumption by determining the structural effect of stereospecific phosphorothioate substitution at five positions in an RNA hairpin containing the binding site for bacteriophage MS2 capsid protein. At most sites, substitution has little or no effect, causing minor perturbations in the phosphate backbone and increasing the stacking among nucleotides in the hairpin loop. At one site, however, phosphorothioate substitution causes an unpaired adenine necessary for formation of the capsid protein-RNA complex to loop out of the RNA helix into the major groove. These results indicate that phosphorothioate substitution can substantially alter the conformation of RNA at positions of irregular secondary structure, complicating the use of substitution-interference experiments to study RNA structure and function.
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The line below this paragraph, {{ABSTRACT_PUBMED_10801314}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 10801314 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10801314}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D0T OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D0T OCA].
==Reference==
==Reference==
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[[Category: Stem-loop]]
[[Category: Stem-loop]]
[[Category: Thiophosphate]]
[[Category: Thiophosphate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:19:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 21:58:48 2008''

Revision as of 18:58, 30 June 2008

Image:1d0t.png

Template:STRUCTURE 1d0t

SOLUTION STRUCTURE OF A PHOSPHOROTHIOATE MODIFIED RNA BINDING SITE FOR PHAGE MS2 COAT PROTEIN

Template:ABSTRACT PUBMED 10801314

About this Structure

Full experimental information is available from OCA.

Reference

Phosphorothioate substitution can substantially alter RNA conformation., Smith JS, Nikonowicz EP, Biochemistry. 2000 May 16;39(19):5642-52. PMID:10801314

Page seeded by OCA on Mon Jun 30 21:58:48 2008

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