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| | ==Solution structure of SPA-2 SHD== | | ==Solution structure of SPA-2 SHD== |
| - | <StructureSection load='6lag' size='340' side='right'caption='[[6lag]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='6lag' size='340' side='right'caption='[[6lag]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6lag]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chrysonilia_crassa Chrysonilia crassa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LAG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LAG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6lag]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LAG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LAG FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">spa2, NCU03115 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5141 Chrysonilia crassa])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lag OCA], [https://pdbe.org/6lag PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lag RCSB], [https://www.ebi.ac.uk/pdbsum/6lag PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lag ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lag OCA], [http://pdbe.org/6lag PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lag RCSB], [http://www.ebi.ac.uk/pdbsum/6lag PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lag ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/V5IQM7_NEUCR V5IQM7_NEUCR] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chrysonilia crassa]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Fan, J S]] | + | [[Category: Neurospora crassa]] |
| - | [[Category: Jedd, G]] | + | [[Category: Fan JS]] |
| - | [[Category: Wong, J Y]] | + | [[Category: Jedd G]] |
| - | [[Category: Yang, D]] | + | [[Category: Wong JY]] |
| - | [[Category: Zheng, P]] | + | [[Category: Yang D]] |
| - | [[Category: Git-pix]]
| + | [[Category: Zheng P]] |
| - | [[Category: Polarity]]
| + | |
| - | [[Category: Shd]]
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| - | [[Category: Signaling protein]]
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| - | [[Category: Spa-2]]
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| Structural highlights
Function
V5IQM7_NEUCR
Publication Abstract from PubMed
The Spitzenkorper (SPK) constitutes a collection of secretory vesicles and polarity-related proteins intimately associated with polarized growth of fungal hyphae. Many SPK-localized proteins are known, but their assembly and dynamics remain poorly understood. Here, we identify protein-protein interaction cascades leading to assembly of two SPK scaffolds and recruitment of diverse effectors in Neurospora crassa. Both scaffolds are transported to the SPK by the myosin V motor (MYO-5), with the coiled-coil protein SPZ-1 acting as cargo adaptor. Neither scaffold appears to be required for accumulation of SPK secretory vesicles. One scaffold consists of Leashin-2 (LAH-2), which is required for SPK localization of the signalling kinase COT-1 and the glycolysis enzyme GPI-1. The other scaffold comprises a complex of Janus-1 (JNS-1) and the polarisome protein SPA-2. Via its Spa homology domain (SHD), SPA-2 recruits a calponin domain-containing F-actin effector (CCP-1). The SHD NMR structure reveals a conserved surface groove required for effector binding. Similarities between SPA-2/JNS-1 and the metazoan GIT/PIX complex identify foundational features of the cell polarity apparatus that predate the fungal-metazoan divergence.
Spitzenkorper assembly mechanisms reveal conserved features of fungal and metazoan polarity scaffolds.,Zheng P, Nguyen TA, Wong JY, Lee M, Nguyen TA, Fan JS, Yang D, Jedd G Nat Commun. 2020 Jun 5;11(1):2830. doi: 10.1038/s41467-020-16712-9. PMID:32503980[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zheng P, Nguyen TA, Wong JY, Lee M, Nguyen TA, Fan JS, Yang D, Jedd G. Spitzenkorper assembly mechanisms reveal conserved features of fungal and metazoan polarity scaffolds. Nat Commun. 2020 Jun 5;11(1):2830. doi: 10.1038/s41467-020-16712-9. PMID:32503980 doi:http://dx.doi.org/10.1038/s41467-020-16712-9
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