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| | <StructureSection load='7c28' size='340' side='right'caption='[[7c28]], [[Resolution|resolution]] 2.40Å' scene=''> | | <StructureSection load='7c28' size='340' side='right'caption='[[7c28]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[7c28]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Denja Denja]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C28 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7C28 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7c28]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dendroaspis_jamesoni_kaimosae Dendroaspis jamesoni kaimosae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C28 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7c28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c28 OCA], [http://pdbe.org/7c28 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7c28 RCSB], [http://www.ebi.ac.uk/pdbsum/7c28 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7c28 ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c28 OCA], [https://pdbe.org/7c28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c28 RCSB], [https://www.ebi.ac.uk/pdbsum/7c28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c28 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/3SIY_DENJA 3SIY_DENJA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Denja]] | + | [[Category: Dendroaspis jamesoni kaimosae]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Jobichen, C]] | + | [[Category: Jobichen C]] |
| - | [[Category: Kini, R M]] | + | [[Category: Kini RM]] |
| - | [[Category: Narumi, A]] | + | [[Category: Narumi A]] |
| - | [[Category: Sivaraman, J]] | + | [[Category: Sivaraman J]] |
| - | [[Category: Synergistic toxin mamba snake venom]]
| + | |
| - | [[Category: Toxin]]
| + | |
| Structural highlights
Function
3SIY_DENJA
Publication Abstract from PubMed
Snake venoms are complex mixtures of enzymes and non-enzymatic proteins that have evolved to immobilize and kill prey animals. Among them, three-finger toxins (3FTxs) belong to the largest superfamily of non-enzymatic proteins. They share a common structure of three b-stranded loops extending like fingers from a central core containing all four conserved disulfide bonds. Most 3FTxs are monomers and through subtle changes in their amino acid sequences they interact with different receptors, ion channels and enzymes to exhibit a wide variety of biological effects. The 3FTxs have further expanded their pharmacological space through covalent or noncovalent dimerization. Synergistic-type toxins (SynTxs) isolated from mamba venoms, highly deadly venomous snake, although nontoxic have been known to enhance toxicity of other venom proteins. However, the details of three-dimensional structure and molecular mechanism of activity of this unusual class of 3FTxs are unclear. We determined the first three-dimensional structure of a SynTx isolated from Dendroaspis jamesoni jamesoni (Jameson's mamba) venom. The SynTx forms a unique homodimer that is held together by an interchain disulfide bond. The dimeric interface is elaborate and encompasses loops II and III. In addition to the inter-subunit disulfide bond, the hydrogen bonds and hydrophobic interactions between the monomers contribute to the dimer formation. Besides, two sulfate ions that mediate interactions between the monomers. This unique quaternary structure is evolved through noncovalent homodimers such as k-bungarotoxins. This novel dimerization further enhances the diversity in structure and function of 3FTxs.
Unusual quaternary structure of a homodimeric synergistic-type toxin from mamba snake venom defines its molecular evolution.,Aoki-Shioi N, Jobichen C, Sivaraman J, Kini RM Biochem J. 2020 Oct 1. pii: 226558. doi: 10.1042/BCJ20200529. PMID:33000863[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Aoki-Shioi N, Jobichen C, Sivaraman J, Kini RM. Unusual quaternary structure of a homodimeric synergistic-type toxin from mamba snake venom defines its molecular evolution. Biochem J. 2020 Oct 1. pii: 226558. doi: 10.1042/BCJ20200529. PMID:33000863 doi:http://dx.doi.org/10.1042/BCJ20200529
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