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| | <StructureSection load='6d0i' size='340' side='right'caption='[[6d0i]], [[Resolution|resolution]] 1.55Å' scene=''> | | <StructureSection load='6d0i' size='340' side='right'caption='[[6d0i]], [[Resolution|resolution]] 1.55Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6d0i]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphyb Sphyb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D0I OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6D0I FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6d0i]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sphingobium_sp._YBL2 Sphingobium sp. YBL2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D0I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6D0I FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TZ53_17660 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=484429 SPHYB]), TZ53_17665 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=484429 SPHYB])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6d0i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d0i OCA], [https://pdbe.org/6d0i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6d0i RCSB], [https://www.ebi.ac.uk/pdbsum/6d0i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6d0i ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6d0i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d0i OCA], [http://pdbe.org/6d0i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d0i RCSB], [http://www.ebi.ac.uk/pdbsum/6d0i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d0i ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/PART_SPHYB PART_SPHYB] Toxic component of a type II toxin-antitoxin (TA) system. Expression in E.coli inhibits cell growth; bacteriostasis is neutralized by expression of cognate antitoxin ParS. ADP-ribosylates E.coli ribose-phosphate pyrophosphokinase (RPPK, prs) using NAD(+) in vitro; ADP-ribosylates RPPK on 'Lys-182' and 'Ser-202'. Cannot use NADP(+). Also auto-ADP-ribosylates in vitro; in the presence of RPPK auto-ADP-ribosylation decreases.<ref>PMID:30598453</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Sphyb]] | + | [[Category: Sphingobium sp. YBL2]] |
| - | [[Category: Jeffrey, P D]] | + | [[Category: Jeffrey PD]] |
| - | [[Category: Link, A J]] | + | [[Category: Link AJ]] |
| - | [[Category: Piscotta, F J]] | + | [[Category: Piscotta FJ]] |
| - | [[Category: Adp-ribosyltransferase]]
| + | |
| - | [[Category: Parst]]
| + | |
| - | [[Category: Toxin]]
| + | |
| - | [[Category: Toxin-antitoxin complex]]
| + | |
| Structural highlights
Function
PART_SPHYB Toxic component of a type II toxin-antitoxin (TA) system. Expression in E.coli inhibits cell growth; bacteriostasis is neutralized by expression of cognate antitoxin ParS. ADP-ribosylates E.coli ribose-phosphate pyrophosphokinase (RPPK, prs) using NAD(+) in vitro; ADP-ribosylates RPPK on 'Lys-182' and 'Ser-202'. Cannot use NADP(+). Also auto-ADP-ribosylates in vitro; in the presence of RPPK auto-ADP-ribosylation decreases.[1]
Publication Abstract from PubMed
Toxin-antitoxin (TA) systems interfere with essential cellular processes and are implicated in bacterial lifestyle adaptations such as persistence and the biofilm formation. Here, we present structural, biochemical, and functional data on an uncharacterized TA system, the COG5654-COG5642 pair. Bioinformatic analysis showed that this TA pair is found in 2,942 of the 16,286 distinct bacterial species in the RefSeq database. We solved a structure of the toxin bound to a fragment of the antitoxin to 1.50 A. This structure suggested that the toxin is a mono-ADP-ribosyltransferase (mART). The toxin specifically modifies phosphoribosyl pyrophosphate synthetase (Prs), an essential enzyme in nucleotide biosynthesis conserved in all organisms. We propose renaming the toxin ParT for Prs ADP-ribosylating toxin and ParS for the cognate antitoxin. ParT is a unique example of an intracellular protein mART in bacteria and is the smallest known mART. This work demonstrates that TA systems can induce bacteriostasis through interference with nucleotide biosynthesis.
ParST is a widespread toxin-antitoxin module that targets nucleotide metabolism.,Piscotta FJ, Jeffrey PD, Link AJ Proc Natl Acad Sci U S A. 2018 Dec 31. pii: 1814633116. doi:, 10.1073/pnas.1814633116. PMID:30598453[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Piscotta FJ, Jeffrey PD, Link AJ. ParST is a widespread toxin-antitoxin module that targets nucleotide metabolism. Proc Natl Acad Sci U S A. 2018 Dec 31. pii: 1814633116. doi:, 10.1073/pnas.1814633116. PMID:30598453 doi:http://dx.doi.org/10.1073/pnas.1814633116
- ↑ Piscotta FJ, Jeffrey PD, Link AJ. ParST is a widespread toxin-antitoxin module that targets nucleotide metabolism. Proc Natl Acad Sci U S A. 2018 Dec 31. pii: 1814633116. doi:, 10.1073/pnas.1814633116. PMID:30598453 doi:http://dx.doi.org/10.1073/pnas.1814633116
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