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6ux5
From Proteopedia
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==Structure of acrorhagin I from the sea anemone Actinia equina== | ==Structure of acrorhagin I from the sea anemone Actinia equina== | ||
| - | <StructureSection load='6ux5' size='340' side='right'caption='[[6ux5]]' scene=''> | + | <StructureSection load='6ux5' size='340' side='right'caption='[[6ux5]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full | + | <table><tr><td colspan='2'>[[6ux5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acteq Acteq]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UX5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UX5 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ux5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ux5 OCA], [https://pdbe.org/6ux5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ux5 RCSB], [https://www.ebi.ac.uk/pdbsum/6ux5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ux5 ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/ACR1_ACTEQ ACR1_ACTEQ]] Toxin.<ref>PMID:16183092</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Acrorhagin I (U-AITX-Aeq5a) is a disulfide-rich peptide identified in the aggressive organs (acrorhagi) of the sea anemone Actinia equina. Previous studies (Toxicon 2005, 46:768-74) found that the peptide is toxic in crabs, although the structural and functional properties of acrorhagin I have not been reported. In this work, an Escherichia coli (BL21 strain) expression system was established for the preparation of (13)C,(15)N-labelled acrorhagin I, and the solution structure was determined using NMR spectroscopy. Structurally, acrorhagin I is similar to B-IV toxin from the marine worm Cerebratulus lacteus (PDB id 1VIB), with a well-defined helical hairpin structure stabilised by four intramolecular disulfide bonds. The recombinant peptide was tested in patch-clamp electrophysiology assays against voltage-gated potassium and sodium channels, and in bacterial and fungal growth inhibitory assays and haemolytic assays. Acrorhagin I was not active against any of the ion channels tested and showed no activity in functional assays, indicating that this peptide may possess a different biological function. Metal ion interaction studies using NMR spectroscopy showed that acrorhagin I bound zinc and nickel, suggesting that its function might be modulated by metal ions or that it may be involved in regulating metal ion levels and their transport. The similarity between the structure of acrorhagin I and that of B-IV toxin from a marine worm suggests that this fold may prove to be a recurring motif in disulfide-rich peptides from marine organisms. | ||
| + | |||
| + | A disulfide-stabilised helical hairpin fold in acrorhagin I: An emerging structural motif in peptide toxins.,Krishnarjuna B, Sunanda P, Villegas-Moreno J, Csoti A, A V Morales R, Wai DCC, Panyi G, Prentis P, Norton RS J Struct Biol. 2020 Dec 30;213(2):107692. doi: 10.1016/j.jsb.2020.107692. PMID:33387653<ref>PMID:33387653</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6ux5" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Acteq]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Krishnarjuna B]] | + | [[Category: Krishnarjuna, B]] |
| - | [[Category: Norton | + | [[Category: Norton, R S]] |
| - | [[Category: Sunanda P]] | + | [[Category: Sunanda, P]] |
| + | [[Category: Toxin]] | ||
Revision as of 14:45, 2 June 2021
Structure of acrorhagin I from the sea anemone Actinia equina
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