6l7z

From Proteopedia

(Difference between revisions)

Revision as of 10:53, 14 June 2023

Solution NMR structure of the N-terminal immunoglobulin variable domain of BTNL2

Structural highlights

6l7z is a 1 chain structure with sequence from Mus musculus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BTNL2_MOUSE Negative regulator of T-cell proliferation.^[1] ^[2]

Publication Abstract from PubMed

T cell costimulation is mediated by the interaction of a number of receptors and ligands present on the surface of the T cell and antigen-presenting cell, respectively. Stimulatory or inhibitory signals from these receptor-ligand interactions work in tandem to preserve immune homeostasis. BTNL2 is a type-1 membrane protein that provides inhibitory signal to T cells and plays an important role in several inflammatory and autoimmune diseases. Therefore, manipulation of the molecular interaction of BTNL2 with its putative receptor could provide strategies to restore immune homeostasis in these diseases. Hence, it is imperative to study the structural characteristics of this molecule, which will provide important insights into its function as well. In this study, the membrane-distal ectodomain of murine BTNL2 was expressed in bacteria as inclusion bodies, refolded in vitro and purified for functional and structural characterization. The domain is monomeric in solution as demonstrated by size-exclusion chromatography and analytical ultracentrifugation, and also binds to its putative receptor on naive B cells and activated T cell subsets. Importantly, for the first time, we report the structure of BTNL2 as determined by solution NMR spectroscopy and also the picosecond-nanosecond timescale backbone dynamics of this domain. The N-terminal ectodomain of BTNL2, which was able to inhibit T cell function as well, exhibits distinctive structural features. The N-terminal ectodomain of BTNL2 has a significantly reduced surface area in the front sheet due to the non-canonical conformation of the CC' loop, which provides important insights into the recognition of its presently unknown binding partner.

Structural Insights into N-terminal IgV Domain of BTNL2, a T Cell Inhibitory Molecule, Suggests a Non-canonical Binding Interface for Its Putative Receptors.,Basak AJ, Maiti S, Hansda A, Mahata D, Duraivelan K, Kundapura SV, Lee W, Mukherjee G, De S, Samanta D J Mol Biol. 2020 Oct 5. pii: S0022-2836(20)30555-6. doi:, 10.1016/j.jmb.2020.09.013. PMID:32976909^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Nguyen T, Liu XK, Zhang Y, Dong C. BTNL2, a butyrophilin-like molecule that functions to inhibit T cell activation. J Immunol. 2006 Jun 15;176(12):7354-60. doi: 10.4049/jimmunol.176.12.7354. PMID:16751379 doi:http://dx.doi.org/10.4049/jimmunol.176.12.7354
↑ Arnett HA, Escobar SS, Gonzalez-Suarez E, Budelsky AL, Steffen LA, Boiani N, Zhang M, Siu G, Brewer AW, Viney JL. BTNL2, a butyrophilin/B7-like molecule, is a negative costimulatory molecule modulated in intestinal inflammation. J Immunol. 2007 Feb 1;178(3):1523-33. PMID:17237401
↑ Basak AJ, Maiti S, Hansda A, Mahata D, Duraivelan K, Kundapura SV, Lee W, Mukherjee G, De S, Samanta D. Structural Insights into N-terminal IgV Domain of BTNL2, a T Cell Inhibitory Molecule, Suggests a Non-canonical Binding Interface for Its Putative Receptors. J Mol Biol. 2020 Oct 5. pii: S0022-2836(20)30555-6. doi:, 10.1016/j.jmb.2020.09.013. PMID:32976909 doi:http://dx.doi.org/10.1016/j.jmb.2020.09.013

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6l7z"

@@ Line 1: / Line 1: @@
 ==Solution NMR structure of the N-terminal immunoglobulin variable domain of BTNL2==
-<StructureSection load='6l7z' size='340' side='right'caption='[[6l7z]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
+<StructureSection load='6l7z' size='340' side='right'caption='[[6l7z]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6l7z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L7Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6L7Z FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6l7z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L7Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L7Z FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Btnl2, Gm315, Ng9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l7z OCA], [https://pdbe.org/6l7z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l7z RCSB], [https://www.ebi.ac.uk/pdbsum/6l7z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l7z ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6l7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l7z OCA], [http://pdbe.org/6l7z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l7z RCSB], [http://www.ebi.ac.uk/pdbsum/6l7z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l7z ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/BTNL2_MOUSE BTNL2_MOUSE]] Negative regulator of T-cell proliferation.<ref>PMID:16751379</ref> <ref>PMID:17237401</ref>
+[https://www.uniprot.org/uniprot/BTNL2_MOUSE BTNL2_MOUSE] Negative regulator of T-cell proliferation.<ref>PMID:16751379</ref> <ref>PMID:17237401</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 22: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Lk3 transgenic mice]]
+[[Category: Mus musculus]]
-[[Category: Basak, A J]]
+[[Category: Basak AJ]]
-[[Category: De, S]]
+[[Category: De S]]
-[[Category: Lee, W]]
+[[Category: Lee W]]
-[[Category: Samanta, D]]
+[[Category: Samanta D]]
-[[Category: Immune system]]
-[[Category: Immunoglobulin fold]]
-[[Category: T cell co-inhibitory molecule]]

6l7z

From Proteopedia

Revision as of 10:53, 14 June 2023

Solution NMR structure of the N-terminal immunoglobulin variable domain of BTNL2

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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